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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 10

sp. nov., a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil No Access

Abstract

An aerobic, Gram-stain-positive and non-spore-forming strain, designated C1-1, was isolated from a fellfield soil sample collected from frost-sorted polygons on Jane Col, Signy Island, Maritime Antarctic. Cells with a size of 0.65–0.9×1.2–1.7 µm have a flagellar motile apparatus and exhibit a rod–coccus growth cycle. Optimal growth conditions were observed at 15–20 °C, pH 7.0 and NaCl concentration up to 0.5 % (w/v) in the medium. The 16S rRNA gene sequence of C1-1 showed the highest pairwise similarity of 98.77 % to NBRC 113092. Phylogenetic trees based on the 16S rRNA and whole-genome sequences revealed that strain C1-1 belongs to the genus and is most closely related to members of the ‘ group’. The G+C content of genomic DNA was 58.95 mol%. The original and orthologous average nucleotide identities between strain C1-1 and NBRC 113092 were 77.15 % and 77.38 %, respectively. The digital DNA–DNA relatedness values between strain C1-1 and NBRC 113092 was 21.6 %. The polar lipid profile was composed mainly of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and an unidentified glycolipid. The predominant cellular fatty acids were anteiso-C (75 %) and anteiso-C (15.2 %). Menaquinone MK-9(H) (86.4 %) was the major respiratory quinone in strain C1-1. The peptidoglycan type was determined as A3α (-Lys–-Ala; A11.6). Based on all described phylogenetic, physiological and chemotaxonomic characteristics, we propose that strain C1-1 (=DSM 112353=CCM 9148) is the type strain of a novel species sp. nov.

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  • Accepted:
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Keyword(s): Antarctica , Arthrobacter , cold-adapted microorganisms , Micrococcaceae and whole-genome sequencing
Funding
This study was supported by the:
  • Center for Geosphere Dynamics, Charles University (Award UNCE/SCI/006)
    • Principle Award Recipient: CajthamlTomas
  • Ministerstvo Školství, Mládeže a Tělovýchovy (Award LM2018131)
    • Principle Award Recipient: UhlikOndrej
  • Ministerstvo Školství, Mládeže a Tělovýchovy (Award LTAUSA19028)
    • Principle Award Recipient: UhlikOndrej
  • Ministerstvo Školství, Mládeže a Tělovýchovy (Award MSMT No 21-SVV/2020)
    • Principle Award Recipient: VodickovaPatricie
  • Ministerstvo Obrany České Republiky (Award 907930101413)
    • Principle Award Recipient: PajerPetr
© 2022 The Authors
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2023-12-06
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Arthrobacter polaris sp. nov., a new cold-adapted member of the family Micrococcaceae isolated from Antarctic fellfield soil
Int J Syst Evol Microbiol 72, 005541 (2022); https://doi.org/10.1099/ijsem.0.005541
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