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Volume 72, Issue 8

sp. nov., isolated from the rhizosphere soil of a decayed mangrove plant No Access

Abstract

In this study, we isolated a novel Gram-stain-positive, aerobic, motile, rod-shaped bacterium, named GX 13764, from the rhizosphere soil of a decayed mangrove plant collected from Beihai, Guangxi, PR China, and characterized using a polyphasic taxonomic approach. The strain exhibited yellow-orange, round, convex, shiny, smooth, opaque and 2–3 mm diameter colonies on marine agar 2216 media after 3 days of incubation at 30 °C and was capable of growth at 4–45 °C (optimum, 30 °C), pH 5.0–9.0 (optimum, pH 7.0) and 0–4 % NaCl (w/v; optimum, 2 %). The strain was positive for catalase and negative for the oxidase. The main cellular fatty acid was anteiso-C, iso-C, iso-C and iso-C. The cell-wall peptidoglycan comprised -diaminopimelic acid and the main menaquinone was MK-7. The polar lipids included one diphosphatidylglycerol, one phosphatidylglycerol, two glycolipids, two unidentified phospholipids and three unidentified lipids. Based on 16S rRNA gene analysis, GX 13764 presented the highest sequence similarity to KCTC 33872 (97.04 %). The DNA G+C content of the type strain was 44.2 mol%. The average nucleotide identity values between GX 13764 and KCTC 33872, DSM 19097 and LMG 22858 were 69.39, 68.87 and 68.95 %, respectively, with digital DNA–DNA hybridization values of 19.9, 19.5 and 19.5 %, respectively. Based on the polyphasic data, strain GX 13764 should be nominated as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GX 13764 (=MCCC 1K06654=KCTC 43366).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Kandelia candel , Metabacillus , new species and rhizosphere soil
© 2022 The Authors
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2022-08-23
2024-05-20
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Metabacillus kandeliae sp. nov., isolated from the rhizosphere soil of a decayed mangrove plant Kandelia candel
Int J Syst Evol Microbiol 72, 005538 (2022); https://doi.org/10.1099/ijsem.0.005538
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