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Volume 72, Issue 10

sp. nov. and sp. nov., two new actinobacterial species exhibiting antimicrobial potential No Access

Abstract

Two novel actinobacterial strains, designated MMS20-R2-23 and MMS20-R2-29, were isolated from riverside soil and subjected to taxonomic characterization. Both strains were Gram-stain-positive, aerobic, non-motile and filamentous, and formed orange to strong orange-brown coloured colonies, which later turned black. Both strains grew optimally at mesophilic temperatures, neutral to slightly alkaline pH and in the absence of NaCl. Analysis of 16S rRNA gene sequences indicated that the two novel strains fell into phylogenetic clusters belonging to the genus . Strains MMS20-R2-23 and MMS20-R2-29 showed the highest 16S rRNA gene sequence similarity to SG15 (99.3 %) and MMS20-R1-14 (99.4 %), respectively. Based on the comparative genome analysis, strain MMS20-R2-23 had the highest orthologous average nucleotide identity (orthoANI) value of 92.70 % with DSM 44100, and MMS20-R2-29 shared 94.99 % with CCTCC AA 2012002. Besides, the digital DNA–DNA hybridization (dDDH) values of MMS20-R2-23 and MMS20-R2-29 with the same species were 47.6 and 59.2% respectively, which were also highest among the compared species, thus confirming the separation of each strain at species level from related species. The orthoANI and dDDH values between MMS20-R2-23 and MMS20-R2-29 were 92.18 and 44.9% respectively. The genomes of strains MMS20-R2-23 and MMS20-R2-29 were estimated as 7.56 Mbp and 7.13 Mbp in size, and the DNA G+C contents were 72.5 and 72.9 mol%, respectively. The chemotaxonomic properties of both strains were consistent with those of the genus. The novel strains showed antimicrobial activity against a broad range of microbes, in particular Gram-positive bacteria and yeasts. It is evident that each of the isolated strains merits recognition as representing novel species of , for which the names sp. nov. (type strain=MMS20-R2-23=KCTC 49542=JCM 34495) and sp. nov. (type strain=MMS20-R2-29=KCTC 49543=JCM 34496) are proposed.

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Keyword(s): antimicrobial potential , biosynthetic gene cluster , Micromonospora antibiotica , Micromonospora humidisoli and riverside soil
© 2022 The Authors
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2022-10-11
2024-04-26
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Micromonospora antibiotica sp. nov. and Micromonospora humidisoli sp. nov., two new actinobacterial species exhibiting antimicrobial potential
Int J Syst Evol Microbiol 72, 005522 (2022); https://doi.org/10.1099/ijsem.0.005522
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