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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 8

sp. nov., an alkaliphilic bacterium isolated from saline-alkaline soil No Access

Abstract

Two alkaliphilic strains, designated APA_J-2 (6-2) and APA_J-5 (13-2), were isolated from saline-alkali soil sampled in Jilin Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two strains APA_J-2 (6-2) and APA_J-5(13–2) were closely related to members of the genus , and had the highest sequence similarity to JCM 17251 (96.8 and 96.9 %, respectively). The 16S rRNA gene sequence similarity between the two novel isolates was 99.6 %, indicating that they were similar species. Cells were Gram-stain-positive, aerobic, motile and rod-shaped. The strains grew at 15–45 °C (optimum, 37 °C), pH 8.0–11.0. (optimum, pH 9) and with 0–10 % (w/v) NaCl (optimum, 5 %). The strains contained menaquinone-7 as the respiratory quinone and anteiso-C as the predominant cellular fatty acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The genomic DNA G+C content was 40.0 mol%. The average nucleotide identity (ANI), amino acid identity (AAI) and digital DNA–DNA hybridization (dDDH) values of strain APA_J-2 (6-2) with JCM 17251 were 85.5, 87.9 and 30.7 %, respectively. The ANI, AAI and dDDH values of strain APA_J-5 (13-2) with JCM 17251 were 85.7, 87.7 and 30.8 %, respectively. Based on the phylogenetic, phenotypic, biochemical, chemotaxonomic and genome data, strains APA_J-2 (6-2) and APA_J-5 (13-2) represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is APA_J-2 (6-2) (=KCTC 43253=GDMCC 1.2242).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Oceanobacillus alkalisoli sp. nov. , polyphasic taxonomy and saline-alkaline soil
Funding
This study was supported by the:
  • Introduction project of high-level talents in Xinjiang Uygur Autonomous Region
    • Principle Award Recipient: Wen-JunLi
  • Introduction project of high-level talents in Xinjiang Uygur Autonomous Region
    • Principle Award Recipient: ShuangWang
  • Key Scientific and Technological Project of Heilongjiang Province of China (Award 2021ZXJ03B05)
    • Principle Award Recipient: WangShuang
  • Projects of the Heilongjiang Academy of Agricultural Sciences (Award 2020FJZX001 and 2021QKPY008)
    • Principle Award Recipient: WangShuang
  • Technical System of national soybean industry (Award CARS-04)
    • Principle Award Recipient: LeiSun
  • National key R&D Program of China MOST (Award 2021YFD1500300)
    • Principle Award Recipient: ShuangWang
© 2022 The Authors

Erratum

An erratum has been published for this content:
Erratum: sp. nov., an alkaliphilic bacterium isolated from saline-alkaline soil
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2022-08-26
2024-05-20
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Oceanobacillus alkalisoli sp. nov., an alkaliphilic bacterium isolated from saline-alkaline soil
Int J Syst Evol Microbiol 72, 005518 (2022); https://doi.org/10.1099/ijsem.0.005518
/content/journal/ijsem/10.1099/ijsem.0.005518
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