1887

Abstract

Several strains of a previously undescribed bacterial species were isolated from mature hybrid trees suffering from bleeding cankers at various geographic locations in the UK. The strains were Gram-negative, facultatively anaerobic, and partial sequencing of the gene revealed that the strains belong to the genus with the closest phylogenetic neighbours being and . Further investigation using a polyphasic approach was undertaken to determine the taxonomic position of the novel species. Phylogenies based on the 16S rRNA gene and multilocus sequence analysis of partial housekeeping gene sequences of , , and revealed that the strains formed an independent cluster within the genus . The phenotypic and chemotaxonomic assays demonstrated that the strains could be differentiated from the closest relatives. Genome analysis of representative strains revealed DNA–DNA hybridization values below the threshold for species delimitation, although the average nucleotide identity values obtained when compared to (95.9–96%) were slightly higher than the suggested cut-off value of 95%. However, as all other data suggests that the strains belong to a novel taxon that can be differentiated from the closest relatives, we propose that the strains represent a novel species in the genus , sp. nov. (type strain WC1b.1=LMG 32575=NCPPB 4697).

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2022-10-07
2024-06-16
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