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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 10

Description and genomic characterization of sp. nov., isolated from No Access

Abstract

Two endophytic bacteria, designated strains CQZ9-1 and MQZ9-1, were isolated from semi-mangrove plant collected from Maowei Sea Mangrove Nature Reserve in Guangxi Zhuang Autonomous Region, PR China. The two strains possessed almost identical 16S rRNA gene sequences (99.7 %). The average nucleotide identity (ANI), average amino acid identity (AAI) and the digital DNA-DNA hybridization (dDDH) values between the two strains were 100 %, indicating that they represented the same species. The 16S rRNA gene sequence similarities between strains CQZ9-1, MQZ9-1 and the most closely related type strains, KSK16Y-1, MQZ13P-4 and CBS 5Q-3 were 98.0–98.1, 97.3–97.4 and 97.3–97.4 %, respectively. The results of phylogenetic analyses based on 16S rRNA gene sequences and genome sequences indicated that CQZ9-1 and MQZ9-1 formed a distinct lineage with CBS5Q-3, 40Bstr34, KSK16Y-1, MQZ13P-4, JCM 30119 and 22II-16-19i. The draft genomes of strains CQZ9-1 and MQZ9-1 were 4  162  933 bp and 4  164  266 bp in size, respectively, and their DNA G+C contents were both 63.8 %. Comparative genome analysis of the two strains and the type strains of related species revealed ANI, AAI and dDDH values below the cut-off levels of 95-96, 95.5 and 70 %, respectively. The ubiquinone detected in CQZ9-1 was Q-10. The major cellular fatty acid of strains CQZ9-1 and MQZ9-1 was found to be Cω7. Combined data from phenotypic, phylogenetic and chemotaxonomic studies indicated that CQZ9-1 and MQZ9-1 represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CQZ9-1 (= CGMCC 1.18725 = JCM 34331).

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Keyword(s): Acrostichum aureum , Jiella flava and novel species
© 2022 The Authors
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2022-10-03
2024-05-18
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Description and genomic characterization of Jiella flava sp. nov., isolated from Acrostichum aureum
Int J Syst Evol Microbiol 72, 005514 (2022); https://doi.org/10.1099/ijsem.0.005514
/content/journal/ijsem/10.1099/ijsem.0.005514
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