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Volume 72, Issue 8

gen. nov., sp. nov., a novel representative of fam. nov. within the order isolated from a grassland soil No Access

Abstract

The order is a deep-branching lineage within the phylum . Most representatives have been isolated from terrestrial environments. A strain isolated from a grassland soil was found to be affiliated with this order and therefore characterized by a polyphasic approach. Cells of strain 0166_1 are Gram-positive, short rods, non-motile, non-spore-forming and divide by binary fission. A surface layer with protrusions covers the majority of the cells. Strain 0166_1 grows optimally around neutral to slightly alkaline pH (pH 7.1–7.9) and at temperatures between 24–36 °C in SSE/HD 1 : 10 medium. It grows optimally with 0–0.5% NaCl (w/v) but can withstand concentrations up to 5 %. The major fatty acids are C ω9, C 7, C cyclo 7, C c methyl and C cyclo 9. The major polar lipids are diphosphatidylglycerol, two unidentified phospholipids and one unidentified glycolipid. MK-7(H4) and MK-7(H2) are the predominant respiratory quinones. -2,6-Diaminopimelic acid is the diagnostic diamino acid in the cell-wall peptidoglycan. The G+C content for strain 0166_1 is 72.8 mol%. 16S rRNA gene sequence analysis indicated that this bacterium was related to KV-962 and YC2-25 with 95.5 and 95.2 % sequence similarity, respectively. Based on the phenotypic, genomic and phylogenetic data, we propose the novel species sp. nov. (type strain 0166_1=DSM 104329=LMG 29999=CECT 9240) of the novel genus gen. nov. within the novel family fam. nov.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Capillimicrobium , halotolerant , high G+C content and soil bacterium
Funding
This study was supported by the:
  • Deutsche Forschungsgemeinschaft (Award OV 20/27)
    • Principle Award Recipient: JörgOvermann
  • Deutsche Forschungsgemeinschaft (Award OV 20/21-1)
    • Principle Award Recipient: JörgOvermann
© 2022 The Authors
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2022-08-26
2024-05-20
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Capillimicrobium parvum gen. nov., sp. nov., a novel representative of Capillimicrobiaceae fam. nov. within the order Solirubrobacterales, isolated from a grassland soil
Int J Syst Evol Microbiol 72, 005508 (2022); https://doi.org/10.1099/ijsem.0.005508
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