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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 8

sp. nov., a Pb-tolerant actinomycete No Access

Abstract

A novel actinobacterium, designated KC 17012, was isolated from lead zinc tailings collected from Lanping, Yunnan, PR China. Comparative 16S rRNA gene sequencing showed that KC 17012 belonged to the genus and was most closely related to the type strains of (98.34%), (98.34%) and (98.27%). Phylogenetic tree analysis revealed strain KC 17012 formed a distinct clade. The genome size was 8.64 Mbp with a DNA G+C content of 70.8%. Digital DNA–DNA hybridization and average nucleotide identity values between the genome sequence of strain KC 17012 and those of JCM 4796 (25.3 and 81.5 %) and NBRC 109811 (30.1 and 85.7 %) were below the thresholds of 70 and 96% for prokaryotic conspecific assignation. The strain formed long straight aerial hyphae which generated regular short rod spores with spiny surfaces. Growth occurred at 10–45 °C, pH 6–8 and with 0–9 % NaCl (w/v). Strain KC 17012 contained -diaminopimelic acid and the major whole-cell hydrolysates included glucose, mannose and ribose. The menaquinones were MK-9(H), MK-9(H) and MK-9(H). The major cellular fatty acids were iso-C, anteiso-C, iso-C and anteiso-C. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, one unidentified lipid and one unidentified phospholipid. On the basis of the results of a polyphasic taxonomic study, it is concluded that KC 17012 represents a novel species of the genus , for which the name sp. nov., is proposed. The type strain is KC 17012 (CGMCC 4.7704=JCM 35204).

  • Received:
  • Accepted:
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Keyword(s): Pb2+ -tolerant , phylogenetic analysis and Streptomyces plumbidurans sp. nov.
Funding
This study was supported by the:
  • The Yunnan Ten Thousand Talents Program for Young Top-Notch Talents (Award YNWR-QNBJ-2018-135)
    • Principle Award Recipient: LiangLian-Ming
  • The Yunnan Ten Thousand Talent Plans for Young Top-notch Talents of Yunnan Province (Award YNWR-QNBJ-2018-011)
    • Principle Award Recipient: CaoYan-Ru
  • A grant from State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University (Award 2019KF005)
    • Principle Award Recipient: CaoYan-Ru
  • National Natural Science Foundation of China (Award 31660002)
    • Principle Award Recipient: CaoYan-Ru
  • Joint Special Project of Universities in Yunnan Province (Award 2018FH001-004)
    • Principle Award Recipient: HouXiu-Li
  • Joint Special Project of Universities in Yunnan Province (Award 2018FH001-003)
    • Principle Award Recipient: CaoYan-Ru
© 2022 The Authors
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Streptomyces plumbidurans sp. nov., a Pb2+-tolerant actinomycete
Int J Syst Evol Microbiol 72, 005505 (2022); https://doi.org/10.1099/ijsem.0.005505
/content/journal/ijsem/10.1099/ijsem.0.005505
/content/journal/ijsem/10.1099/ijsem.0.005505
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