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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 8

sp. nov., a moderately halophilic bacterium isolated from a grey saltern No Access

Abstract

A novel moderately halophilic bacterium, designated strain KHM2, was isolated from the sediment of a grey solar saltern located on Sinui Island, Shinan, Republic of Korea. Cells were rod-shaped, endospore-forming, Gram-stain-positive, motile and facultative anaerobic. Strain KHM2 performed anaerobic respiration using nitrates and did not produce glucose acids, indicating the absence of fermentation. Strain KHM2 grew at 10–45 °C (optimum, 37 °C), pH 6.0–10.0 (optimum, pH 8.0) and with 1.0–20.0 % (w/v) NaCl (optimum, 10.0%). Based on 16S rRNA gene sequence similarity and chemotaxonomic properties, strain KHM2 was assigned to the genus , with high 16S rRNA gene sequence similarity to B6B (98.2%) and less than 96.8 % similarity to the other recognized members of the genus . The polar lipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylglycerol (PG) and one unidentified phospholipid (PL). Major fatty acids were anteiso-C and anteiso-C. The average nucleotide identity and digital DNA–DNA hybridization values of strain KHM2 with B6B were 77.6 and 22.0 %, respectively. Based on the results of polyphasic analysis, strain KHM2 is proposed to represent a bacterial species within the genus with the name sp. nov. The type strain is KHM2 (=KACC 19068=NBRC 112577)

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Keyword(s): aquibacillus , grey saltern , moderately halophilic bacterium and taxonomy
© 2022 The Authors
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2022-08-22
2024-05-20
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Aquibacillus saliphilus sp. nov., a moderately halophilic bacterium isolated from a grey saltern
Int J Syst Evol Microbiol 72, 005496 (2022); https://doi.org/10.1099/ijsem.0.005496
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