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Volume 72, Issue 8

sp. nov. and sp. nov., isolated from flowers No Access

Abstract

Two aerobic, Gram-stain-negative, motile, mesophilic, rod-shaped and catalase-positive bacterial strains designated AF9R3 and GN2-R2 were isolated from flowers collected in the Republic of Korea. Strain AF9R3 grew at 4–33 °C, pH 4.0–9.0 and with 0–1 % NaCl (w/v), and strain GN2-R2 grew at 10–33 °C, pH 4.0–9.0 and with 0–1 % NaCl (w/v). Phylogenetic analysis on the basis of 16S rRNA gene sequences indicated that strains AF9R3 and GN2-R2 belonged to the genera and , respectively, showing high sequence similarity to CY42W (99.4 %) and 6 NM-7 (98.0 %), respectively. Both strains contained summed feature 3 (C 7 and/or C 6) and C as the major fatty acids, and ubiquinone Q-8 as the predominant quinone. Strain AF9R3 had diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, and strain GN2-R2 comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid as the major polar lipids. Orthologous average nucleotide identity and digital DNA–DNA hybridization values of strain AF9R3 to its closest relative CY42W were 92.6 and 56.5 %, and those of strain GN2-R2 to its closest relative 6 NM-7 were 81.4 and 24.8 %. Based on genotypic and phenotypic data, strains AF9R3 and GN2-R2 are considered to represent novel species of the genus and , respectively, for which the names sp. nov. (type strain AF9R3=KACC 21258=NBRC 114510) and sp. nov. (type strain GN2-R2=KACC 21261=NBRC 114511) have been proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): menaquinone , MIDI , novel species , polar lipid , strain AF9R3T and strain GN2-R2T
© 2022 The Authors
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2022-08-16
2024-04-30
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Duganella dendranthematis sp. nov. and Massilia forsythiae sp. nov., isolated from flowers
Int J Syst Evol Microbiol 72, 005487 (2022); https://doi.org/10.1099/ijsem.0.005487
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