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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 72, Issue 8

sp. nov., a psychrotrophic anaerobic bacterium isolated from rice field soil Free

Abstract

A cold-adapted or psychrotrophic anaerobic bacterial strain (C5S11) was isolated from rice field soil in Japan using an enrichment culture incubated at 5 °C. C5S11 grew at 0 °C and optimum growth was observed at 10 °C. Cells of C5S11 were Gram-stain-positive, motile, spore-forming rods with peritrichous flagella. C5S11 was assigned to a large branch consisted of various mesophilic species of the genus in the phylogenetic trees reconstructed using the 16S rRNA gene sequences. The most closely related species of the strain was DSM 23318 (98.5 % sequence similarity). C5S11 fermented various carbohydrates, including polysaccharides (starch, inulin, pectin and xylan), and produced acetate, butyrate and H as major products. The major cellular fatty acids were C, Cω9, C and Cω7. The diagnostic diamino acid of the cell wall peptidoglycan was -diaminopimelic acid. The genome size of C5S11 was 6.04 Mb and the genomic DNA G+C content was 29.0 mol%. Average nucleotide identity by (ANIb), avaerage amino acid identity (AAI) and digital DNA–DNA hybridization (dDDH) values between the genomes of C5S11 and DSM 23318 were 80.0, 75.2 and 26.4 %, respectively. C5S11 had a gene encoding cold shock protein (RNA chaperone) in the genome, homologues of which have been found in psychrophilic species of the genus . On the basis of the differences in the phylogenetic, genomic and phenotypic characteristics of C5S11 from those of the closely related species, a novel species, sp. nov., is proposed to accommodate the strain. The type strain is C5S11 (=NBRC 114689 = DSM 112608).

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Keyword(s): anaerobic Gram-positive rods , Clostridium estertheticum , Clostridium gelidum , cold-adapted bacteria and psychrophillic
Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award JP15580295)
    • Principal Award Recipient: AtsukoUeki
© 2022 The Authors
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2022-08-23
2025-11-11

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Clostridium gelidum sp. nov., a psychrotrophic anaerobic bacterium isolated from rice field soil
Int J Syst Evol Microbiol 72, 005478 (2022); https://doi.org/10.1099/ijsem.0.005478
/content/journal/ijsem/10.1099/ijsem.0.005478
/content/journal/ijsem/10.1099/ijsem.0.005478
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