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Volume 72, Issue 8

Genome-based reclassification of Az39 as the type strain of sp. nov. No Access

Abstract

Strain Az39 of is a diazotrophic plant growth-promoting bacterium isolated in 1982 from the roots of wheat plants growing in Marcos Juárez, Córdoba, Argentina. It produces indole-3-acetic acid in the presence of -tryptophan as a precursor, grows at 20–38 °C (optimal 38 °C), and the cells are curved or spiral-shaped, with diameters ranging from 0.5–0.9 to 1.8–2.2 µm. They contain C, C and C 7/6 as the main fatty acids. Phylogenetic analysis of its 16S rRNA gene sequence confirmed that this strain belongs to the genus , showing a close relationship with Sp245, Sp7 and CC-Nfb-7. Housekeeping gene analysis revealed that Az39, together with five strains of the genus (Az19, REC3, BR 11975, MTCC4035 and MTCC4036), form a cluster apart from Sp245, Sp7 and CC-Nfb-7. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) between Az39 and the aforementioned type strains revealed values below 96 %, the circumscription limit for the species delineation (ANI: 95.3, 94.1 and 94.0 %; dDDH: 62.9, 56.3 and 55.6 %). Furthermore, a phylogeny evaluation of the core proteome, including 809 common shared proteins, showed an independent grouping of Az39, Az19, REC3, BR 11975, MTCC4035 and MTCC4036. The G+C content in the genomic DNA of these six strains varied from 68.3 to 68.5 %. Based on the combined phylogenetic, genomic and phenotypic characterization presented here, we consider that strain Az39, along with strains Az19, REC3, BR 11975, MTCC4035 and MTCC4036, are members of a new species, for which the name sp. nov. is proposed. The type strain is Az39 (=LBPCV39=BR 148428=CCCT 22.01).

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Keyword(s): proteome-core , Azospirillum , genome , housekeeping genes and phylogenies
© 2022 The Authors
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2022-08-01
2024-05-02
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Genome-based reclassification of Azospirillum brasilense Az39 as the type strain of Azospirillum argentinense sp. nov.
Int J Syst Evol Microbiol 72, 005475 (2022); https://doi.org/10.1099/ijsem.0.005475
/content/journal/ijsem/10.1099/ijsem.0.005475
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