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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 7

f.a., sp. nov., a new ascomycetous yeast species in the clade isolated in Taiwan No Access

Abstract

This study describes , a new anamorphic ascomycetous yeast species, four strains of which were isolated from soil and the fruiting body of a mushroom in Taiwan between 2006 and 2007. Analysis of the sequences of the large-subunit rRNA, small-subunit rRNA and elongation factor-1α identified this species as a member of the clade. The yeast strains of exhibited a 0–3 nucleotide difference in the sequences of the D1/D2 domain of the large subunit rRNA when compared to one another and a 10 and 11 nucleotide difference when compared to sp. BG99-11-14-10-4-1 and NRRL Y-7574, the closest undescribed species, respectively. The yeast strains differed by 77 and 78 nucleotides from s and , the close species, respectively. The internal transcribed spacer sequences of the four isolates exhibited a divergence of 106–108 substitutions from the recognized species . No sexual reproduction was observed. The strains differed from those of related species in terms of their carbon and nitrogen assimilation patterns. Therefore, this study proposes f.a., sp. nov. to accommodate these four strains, with BCRC 23185 (isotype CBS 11432; MycoBank number MB563484) as the holotype.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): new species , phylogenetic analysis , Taiwan and Wickerhamomyces
Funding
This study was supported by the:
  • Ministry of Science and Technology (Award MOST 110-2621-B-007-001)
    • Principle Award Recipient: Ching-FuLee
  • Ministry of Science and Technology (Award MOST 110-2637-B-157-001)
    • Principle Award Recipient: Chin-FengChang
  • Ministry of Natural Resources (Award MOST 104-2621-B-134 -001-MY3)
    • Principle Award Recipient: Ching-FuLee
  • Ministry of Science and Technology (Award NSC 101-2621-B-134-001-MY3)
    • Principle Award Recipient: Ching-FuLee
  • Ministry of Science and Technology (Award NSC 97-2621-B-134-001-MY3)
    • Principle Award Recipient: Ching-FuLee
© 2022 The Authors
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2022-07-22
2024-05-01
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References

  1. Kurtzman CP. Pichia EC Hansen emend. Kurtzman. In Kurtzman CP, Fell JW. eds The Yeasts, a Taxonomic Study, 4th edn. Amsterdam: Elsevier; 1998 pp 273–352
     [Google Scholar]
  2. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek 1998; 73:331–371 [View Article] [PubMed]
     [Google Scholar]
  3. Kurtzman CP, Robnett CJ, Basehoar-Powers E. Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov. FEMS Yeast Res 2008; 8:939–954 [View Article] [PubMed]
     [Google Scholar]
  4. Minter DW. Cyberlindnera, a replacement name for Lindnera Kurtzman et al., nom. illegit. Mycotaxon 2009; 110:473–476
     [Google Scholar]
  5. Chai CY, Huang LN, Cheng H, Liu WJ, Hui FL. Wickerhamomyces menglaensis f.a., sp. nov., a yeast species isolated from rotten wood. Int J Syst Evol Microbiol 2019; 69:1509–1514 [View Article] [PubMed]
     [Google Scholar]
  6. Zhou Y, Jia B-S, Han P-J, Wang Q-M, Li A-H et al. Wickerhamomyces kurtzmanii sp. nov. an ascomycetous yeast isolated from crater lake water, Da Hinggan Ling Mountain, China. Curr Microbiol 2019; 76:1537–1544 [View Article]
     [Google Scholar]
  7. Shimizu Y, Konno Y, Tomita Y. Wickerhamomyces psychrolipolyticus f.a., sp. nov., a novel yeast species producing two kinds of lipases with activity at different temperatures. Int J Syst Evol Microbiol 2020; 70:1158–1165 [View Article] [PubMed]
     [Google Scholar]
  8. Brysch-Herzberg M, Dlauchy D, Seidel M, Péter G. Cyberlindnera sylvatica sp. nov., a yeast species isolated from forest habitats. Int J Syst Evol Microbiol 2021; 71: [View Article] [PubMed]
     [Google Scholar]
  9. Yurkov AM, Schäfer AM, Begerow D. Barnettozyma vustinii A. Yurkov, AM Schäfer & Begerow, sp. nov. Persoonia 2009; 38:188–189
     [Google Scholar]
  10. Imanishi Y, Yamazaki A, Nakase T. A new Barnettozyma species forming hat-shaped ascospores isolated from soil in Japan. J Gen Appl Microbiol 2010; 56:447–453 [View Article] [PubMed]
     [Google Scholar]
  11. Polburee P, Yongmanitchai W, Ohashi T, Fujiyama K, Limtong S. Barnettozyma siamensis f.a., sp. nov., a lipid-accumulating ascomycete yeast species. Int J Syst Evol Microbiol 2014; 64:3053–3057 [View Article] [PubMed]
     [Google Scholar]
  12. Kobayashi R, Kanti A, Kawasaki H. Three novel species of d-xylose-assimilating yeasts, Barnettozyma xylosiphila sp. nov., Barnettozyma xylosica sp. nov. and Wickerhamomyces xylosivorus f.a., sp. nov. Int J Syst Evol Microbiol 2017; 67:3971–3976 [View Article]
     [Google Scholar]
  13. Arrey G, Li G, Murphy R, Guimaraes L, Alizadeh S et al. Isolation, characterization, and genome assembly of Barnettozyma botsteinii sp. nov. and novel strains of Kurtzmaniella quercitrusa isolated from the intestinal tract of the termite Macrotermes bellicosus. G3 (Bethesda) 2021; 11:jkab342 [View Article] [PubMed]
     [Google Scholar]
  14. Kurtzman CP, Robnett CJ, Basehoar-Powers E. Wickerhamomyces. In Kurtzman CP, Fell JW, Boekhout T. eds The Yeasts, a Taxonomic Study, 5th edn. Amsterdam: Elsevier; 2011 pp 899–917
     [Google Scholar]
  15. Zheng J, Lu YF, Liu XJ, Hui FL. Cyberlindnera xishuangbannaensis f.a., sp. nov., a yeast isolated from rotting wood. Int J Syst Evol Microbiol 2017; 67:5051–5055 [View Article] [PubMed]
     [Google Scholar]
  16. Lee CF, Liu CH, Young SS, Chang KS. Kazachstania jiainicus sp. nov., an ascomycetous yeast species isolated from soil in Taiwan. FEMS Yeast Res 2008; 8:114–118 [View Article] [PubMed]
     [Google Scholar]
  17. Hsieh C-W, Huang L-Y, Tschen EF-T, Chang C-F, Lee C-F. Five novel anamorphic, ascomycetous yeast species associated with mushrooms and soil. FEMS Yeast Res 2010; 10:948–956 [View Article] [PubMed]
     [Google Scholar]
  18. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, Fell JW, Boekhout T. eds The Yeasts, a Taxonomic Study, 5th edn. Amsterdam: Elsevier; 2011 pp 137–144
     [Google Scholar]
  19. Kurtzman CP, Robnett CJ. Phylogenetic relationships among yeasts of the “Saccharomyces complex” determined from multigene sequence analyses. FEMS Yeast Res 2003; 3:417–432 [View Article] [PubMed]
     [Google Scholar]
  20. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. eds PCR Protocols: A Guide for Methods and Applications New York: Academic Press; 1990 pp 315–322
     [Google Scholar]
  21. Kurtzman CP, Albertyn J, Basehoar-Powers E. Multigene phylogenetic analysis of the Lipomycetaceae and the proposed transfer of Zygozyma species to Lipomyces and Babjevia anomala to Dipodascopsis. FEMS Yeast Res 2007; 7:1027–1034 [View Article] [PubMed]
     [Google Scholar]
  22. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article] [PubMed]
     [Google Scholar]
  23. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article] [PubMed]
     [Google Scholar]
  24. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article] [PubMed]
     [Google Scholar]
  25. Knutsen AK, Robert V, Poot GA, Epping W, Figge M et al. Polyphasic re-examination of Yarrowia lipolytica strains and the description of three novel Candida species: Candida oslonensis sp. nov., Candida alimentaria sp. nov. and Candida hollandica sp. nov. Int J Syst Evol Microbiol 2007; 57:2426–2435 [View Article]
     [Google Scholar]
  26. Ko KS, Jung HS. Three nonorthologous ITS1 types are present in a polypore fungus Trichaptum abietinum. Mol Phylogenet Evol 2002; 23:112–122 [View Article] [PubMed]
     [Google Scholar]
  27. Vu D, Groenewald M, Szöke S, Cardinali G, Eberhardt U et al. DNA barcoding analysis of more than 9 000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation. Stud Mycol 2016; 85:91–105 [View Article] [PubMed]
     [Google Scholar]
  28. de García V, Brizzio S, Libkind D, Rosa CA, van Broock M. Wickerhamomyces patagonicus sp. nov., an ascomycetous yeast species from Patagonia, Argentina. Int J Syst Evol Microbiol 2010; 60:1693–1696 [View Article] [PubMed]
     [Google Scholar]
  29. Kaewwichian R, Kawasaki H, Limtong S. Wickerhamomyces siamensis sp. nov., a novel yeast species isolated from the phylloplane in Thailand. Int J Syst Evol Microbiol 2013; 63:1568–1573 [View Article] [PubMed]
     [Google Scholar]
  30. Francesca N, Carvalho C, Almeida PM, Sannino C, Settanni L et al. Wickerhamomyces sylviae f.a., sp. nov., an ascomycetous yeast species isolated from migratory birds. Int J Syst Evol Microbiol 2013; 63:4824–4830 [View Article] [PubMed]
     [Google Scholar]
  31. Masiulionis VE, Pagnocca FC. Wickerhamomyces spegazzinii sp. nov., an ascomycetous yeast isolated from the fungus garden of Acromyrmex lundii nest (Hymenoptera: Formicidae). Int J Syst Evol Microbiol 2016; 66:2141–2145 [View Article] [PubMed]
     [Google Scholar]
  32. Suh SO, McHugh JV, Blackwell M. Expansion of the Candida tanzawaensis yeast clade: 16 novel Candida species from basidiocarp-feeding beetles. Int J Syst Evol Microbiol 2004; 54:2409–2429 [View Article] [PubMed]
     [Google Scholar]
  33. Lachance MA, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP. Candida Berkhout (1923). In Kurtzman CP, Fell JW, Boekhout T. eds The Yeasts, a Taxonomic Study, 5th edn. Amsterdam: Elsevier; 1923 pp 987–1278
     [Google Scholar]
  34. Kurtzman CP. Four new yeasts in the Pichia anomala clade. Int J Syst Evol Microbiol 2000; 50 Pt 1:395–404 [View Article] [PubMed]
     [Google Scholar]
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Wickerhamomyces sinyiensis f.a., sp. nov., a new ascomycetous yeast species in the Wickerhamomyces clade isolated in Taiwan
Int J Syst Evol Microbiol 72, 005466 (2022); https://doi.org/10.1099/ijsem.0.005466
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