1887

Abstract

During an investigation of the diversity of airborne yeasts in a famous Chinese baijiu fermentation workshop, two yeast strains were isolated from the air of the Wuliangye 501# baijiu-making workshop in Yibin, Sichuan Province, PR China and subjected to taxonomic analysis. The results of phylogenetic analysis of two regions of the rRNA gene cluster, the D1/D2 domains of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, indicated that these strains represented a novel species of the genus distinct from closely related species. The similarity between the novel species and the most closely related known species, , was 94.1 % for the D1/D2 LSU rRNA gene (30 substitutions and 12 indels out of 556 bp) and 95.6 % for the ITS region (9 substitutions and 6 indels out of 475 bp). In contrast with , this novel species was able to assimilate -ribose, -arabinose, -arabinose, cellobiose, -glucono-1,5-lactone, -lactate, citrate, 1,2-propanediol, 2,3-butanediol and ethanol but was unable to ferment raffinose or assimilate inulin. On the basis of the results of phylogenetic analysis and the physiological characteristics, these investigated strains represent a novel species of the genus , for which the name sp. nov. is proposed. Its holotype is CGMCC 20235, and the MycoBank number is MB840188.

Funding
This study was supported by the:
  • Yibin High-tech program (Award 2021GH001)
    • Principle Award Recipient: JiaZheng
  • Solid-state Fermentation Resource Utilization Key Laboratory of Sichuan Province (Award 2021GTJC02)
    • Principle Award Recipient: XuejunLei
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/content/journal/ijsem/10.1099/ijsem.0.005464
2022-07-20
2024-12-02
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References

  1. Luo FW, Huang YG, Tu HB. Structure and diversity of culturable yeast populations in different Maotai-flavor liquor brewing regions of Maotai town, Guizhou province. Food Sci 2020; 41:143–149
    [Google Scholar]
  2. Wang Q-M, Theelen B, Groenewald M, Bai F-Y, Boekhout T et al. Moniliellomycetes and Malasseziomycetes, two new classes in Ustilaginomycotina. Persoonia 2014; 33:41–47 [View Article] [PubMed]
    [Google Scholar]
  3. Rosa CA, Jindamorakot S, Limtong S, Nakase T, Lachance M-A et al. Synonymy of the yeast genera Moniliella and Trichosporonoides and proposal of Moniliella fonsecae sp. nov. and five new species combinations. Int J Syst Evol Microbiol 2009; 59:425–429 [View Article] [PubMed]
    [Google Scholar]
  4. Stolk AC, Dakin JC. Moniliella, a new genus of Moniliales. Antonie Van Leeuwenhoek 1966; 32:399–409 [View Article] [PubMed]
    [Google Scholar]
  5. de Hoog GS, Smith MT, Rosa CA. Moniliella Stolk & Dakin (1966). In Kurtzman CP, Fell JW, Boekhout T. eds The Yeasts, A Taxonomic Study, 5th ed. London: Elsevier; 2011 pp 1837–1846
    [Google Scholar]
  6. Thanh VN, Hai DA, Hien DD, Takashima M, Lachance MA. Moniliella carnis sp. nov. and Moniliella dehoogii sp. nov., two novel species of black yeasts isolated from meat processing environments. Int J Syst Evol Microbiol 2012; 62:3088–3094 [View Article] [PubMed]
    [Google Scholar]
  7. Thanh VN, Duc Hien D, Yaguchi T, Sampaio JP, Lachance M-A et al. Moniliella sojae sp. nov., a species of black yeasts isolated from Vietnamese soy paste (tuong), and reassignment of Moniliella suaveolens strains to Moniliella pyrgileucina sp. nov., Moniliella casei sp. nov. and Moniliella macrospora emend. comb. nov. Int J Syst Evol Microbiol 2018; 68:1806–1814 [View Article] [PubMed]
    [Google Scholar]
  8. Thanh VN, Hien DD, Thom TT. Moniliella byzovii sp. nov., a chlamydospore-forming black yeast isolated from flowers. Int J Syst Evol Microbiol 2013; 63:1192–1196 [View Article] [PubMed]
    [Google Scholar]
  9. Thanh VN, Hien DD. Moniliella floricola sp. nov., a species of black yeast isolated from flowers. Int J Syst Evol Microbiol 2019; 69:87–92 [View Article] [PubMed]
    [Google Scholar]
  10. Thoa VK, Khanh N, Hien DD, Manh LD et al. Screening of Moniliella yeast with high-level production of erythritol. In Proceedings of International Scientific - Practical Conference "Food, Technologies & Health Plovdiv, Bulgaria: 2015 pp 129–134
    [Google Scholar]
  11. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 1998; 73:331–371 [View Article] [PubMed]
    [Google Scholar]
  12. White TJ, Bruns TD, Lee SB, Taylor JW. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In Innis MA, Gelfand DH, Sninsky JJ, White TJ. eds PCR Protocols: A Guide to Methods and Applications London: Academic Press; 1990 pp 315–322
    [Google Scholar]
  13. Rehner SA, Buckley E. A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 2005; 97:84–98 [View Article] [PubMed]
    [Google Scholar]
  14. Carbone I, Kohn LM. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 2019; 91:553–556 [View Article]
    [Google Scholar]
  15. Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 25:3389–3402 [View Article] [PubMed]
    [Google Scholar]
  16. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article] [PubMed]
    [Google Scholar]
  17. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article] [PubMed]
    [Google Scholar]
  18. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article] [PubMed]
    [Google Scholar]
  19. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article] [PubMed]
    [Google Scholar]
  20. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In Kurtzman CP, JW F, Boekhout T. eds The Yeasts – A Taxonomic Study, 5th ed. Amsterdam: Elsevier; 2011 pp 87–110
    [Google Scholar]
  21. Yarrow D. Method for the isolation, maintenance and identification of yeasts. In Kurtzman CP, Fell JW. eds The Yeasts – A Taxonomic Study, 4th edn. Elsevier, Amsterdam: 1998 pp 77–100
    [Google Scholar]
  22. Mesbah M, Premachandran U, Whitman WB. Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 1989; 39:159–167 [View Article]
    [Google Scholar]
  23. Bai F, Liu Y, Li N, Yao S, Li N et al. Microsporomyces hainanensis sp. nov., isolated from hybrid rice (Oryza sativa L.) seeds. Curr Microbiol 2016; 73:569–573 [View Article] [PubMed]
    [Google Scholar]
  24. Vu D, Groenewald M, Szöke S, Cardinali G, Eberhardt U et al. DNA barcoding analysis of more than 9 000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation. Stud Mycol 2016; 85:91–105 [View Article] [PubMed]
    [Google Scholar]
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