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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 7

sp. nov., a novel member of the genus , isolated from Rainbow Trout () No Access

Abstract

A strain, S-1 was isolated from rainbow trout () exhibiting clinical symptoms of lens atrophy, inappetence, visual impairment and growth retardation. The strain was identified as representing a member of the genus on the basis of the results of 16S rRNA gene sequence analysis. The neighbor-joining phylogenetic tree based on 16S rRNA gene sequences indicated that S-1 clustered with JCM 20190, DSM 15900, and P010, sharing 99.3, 98.8 and 87.7% 16S rRNA gene similarities, respectively. A polyphasic taxonomic approach including phenotypic, chemotaxonomic, and genomic characterization was employed to ascertain the taxonomic position of S-1 within the genus . The overall genome relatedness indices (OGRI) for S-1 compared with the most closely related type strains ATCC BAA-1207, NCTC 10735, ATCC 8071 and DSM 15900 were calculated as 40.8, 40.1, 28.5 and 27.3% for digital DNA–DNA hybridization (dDDH), and 91.6, 91.0, 86.3 and 85.1% for average nucleotide identity (ANI), respectively. OGRI values between S-1 and its close neighbours confirmed that the strain represents a novel species in the genus .The DNA G+C content of the strain is 45.2%. Major fatty acids were Cω8, Ciso, and summed feature 3 (Cω6 and/or Cω7). The predominant polar lipids were phosphatidylethanolamine, phospholipid, amino-phospholipid and unidentified lipids. The major respiratory quinones were ubiquinone-8, ubiquinone-7 and menaquinone-7. Chemotaxonomic and phylogenomic analyses of this isolate confirmed that the strain represents a novel species for which the name sp. nov. is proposed, with S-1 as the type strain (JCM 34183= KCTC 82249).

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  • Accepted:
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Keyword(s): aquaculture , novel species , overall genome-related index , rainbow trout and Shewanella
© 2022 The Authors
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2022-07-20
2024-04-28
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Shewanella oncorhynchi sp. nov., a novel member of the genus Shewanella, isolated from Rainbow Trout (Oncorhynchus mykiss)
Int J Syst Evol Microbiol 72, 005460 (2022); https://doi.org/10.1099/ijsem.0.005460
/content/journal/ijsem/10.1099/ijsem.0.005460
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