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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 8

sp. nov., isolated from red alga No Access

Abstract

A Gram-stain-negative and rod-shaped bacterial strain (WSW3-B6) was isolated from red alga collected from the West Sea, Republic of Korea. Cells of strain WSW3-B6 were non-motile, aerobic and produced slightly yellow and mucoid colonies on marine agar. The strain grew optimally at 23–30 °C, with 0.5–4 % NaCl (w/v) and at pH 6.5–8.5. A phylogenetic analysis of the 16S rRNA gene revealed that strain WSW3-B6 belongs to the genus within the family , having the highest sequence similarity to SM1502 (96.7%), followed by subsp. LaA7.5 (96.2%) and subsp. SaA2.12 (96.2%). The complete sequence of a circular chromosome of strain WSW3-B6 determined by combination of Oxford Nanopore and Illumina platforms comprised a total 2 725 095 bp with G+C content of 37.1 mol%. A comparative analysis based on the whole genome also showed the distinctiveness of strain WSW3-B6. The average nucleotide identity (ANI) values between strain WSW3-B6 and the closest strains SM1502, subsp. LaA7.5 and subsp. SaA2.12 were 78.3, 77.8 and 77.7 %, respectively, while the digital DNA–DNA hybridization (dDDH) values between strain WSW3-B6 and the above closely related strains were 21.0, 20.4 and 20.3 %, respectively. Both the ANI and dDDH values supported the creation of a new species in the genus . The major fatty acids (>10 %) were iso-C (19.3 %), C (14.0 %), iso-C 3-OH (13.1 %) and C (10.7 %). The polar lipids of strain WSW3-B6 included phosphatidylethanolamine, three unidentified aminolipids and three unidentified lipids. Moreover, MK-6 was the only respiratory quinone. A comparison of the phylogenetic distinctiveness and the unique phenotypic and chemotaxonomic characteristics among strain WSW3-B6 and closely related type strains supported that strain WSW3-B6 (=KCTC 82708=GDMCC 1.2627) represents a novel species of the genus , for which the name sp. nov. is proposed.

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Keyword(s): bacteria , Bacteroidota and seaweed
© 2022 The Authors
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2022-08-11
2024-05-20
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Flavobacterium litorale sp. nov., isolated from red alga
Int J Syst Evol Microbiol 72, 005458 (2022); https://doi.org/10.1099/ijsem.0.005458
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