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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 7

sp. nov., isolated from a soil sample of glacier foreland in Austre Lovénbreen, Arctic No Access

Abstract

A rod-shaped, Gram-stain-negative, non-motile and aerobic bacterium, designated Q8-18, was isolated from soil of glacier foreland in Austre Lovénbreen, Arctic, and subjected to a polyphasic taxonomic study. Strain Q8-18 grew optimally at 20 °C, pH 5.0–8.0 and in the presence of 0–1.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain Q8-18 belonged to the family . Strain Q8-18 showed the highest sequence similarity to CCM 8685 (96.8%), CCM 8691 (96.8%), CL-GP80 (96.7%), E01020 (96.7%), DSM 19624 (96.6%), THG-DR3 (96.3%), DCY49 (95.9%) and CCM 8689 (95.9%). A whole genome-level comparison of strain Q8-18 with CL-GP80, E01020, DSM 19624 and LMG 10339 revealed average nucleotide identity values of 77.0, 76.0, 77.0 and 70.4%, respectively. The only respiratory isoprenoid quinone was menaquinone-7. The polar lipid profile of strain Q8-18 was found to contain one phosphatidylethanolamine, eight unidentified aminolipids, one aminophospholipids and five unidentified lipids. The G+C content of the genomic DNA was determined to be 35.4 mol%. The main fatty acids were summed feature 3 (comprising C 7 and/or C 6), iso-C and anteiso-C. On the basis of the evidence presented in this study, a novel species of the genus , sp. nov., is proposed, with the type strain Q8-18 (=CCTCC AB 2020009=KCTC 82636).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): novel species , Pedobacter mucosus , phylogenetic analysis and taxonomy
Funding
This study was supported by the:
  • the Chinese Polar Scientific Strategy Research Fund (Award IC201706)
    • Principle Award Recipient: FangPeng
  • the National Natural Science Foundation of China (Award Grant No. 42076230)
    • Principle Award Recipient: FangPeng
  • R&D Infrastructure and Facility Development Program of the Ministry of Science and Technology of the People’s Republic of China (Award Grant No. NIMR-2020-8)
    • Principle Award Recipient: FangPeng
  • National Key R&D Program of China (Award 2018YFC1406701)
    • Principle Award Recipient: FangPeng
© 2022 The Authors
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2022-07-12
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Pedobacter mucosus sp. nov., isolated from a soil sample of glacier foreland in Austre Lovénbreen, Arctic
Int J Syst Evol Microbiol 72, 005448 (2022); https://doi.org/10.1099/ijsem.0.005448
/content/journal/ijsem/10.1099/ijsem.0.005448
/content/journal/ijsem/10.1099/ijsem.0.005448
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