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Volume 72, Issue 6

sp. nov. and sp. nov., two actinobacteria isolated from saline soil, and reclassification of as comb. nov. No Access

Abstract

Two Gram-stain-positive, non-motile, strictly aerobic, yellow-coloured, rod-shaped bacterial strains, designated LR1S40 and M4N3S171, were isolated from rhizosphere and bulk saline soil of collected in Inner Mongolia, China. Phylogenetic trees based on 16S rRNA gene and whole genome sequences showed that the two strains clustered tightly with strains of the genus . Strains LR1S40 and M4N3S171 had 95.5% 16S rRNA gene similarity to each other, and strain LR1S40 had 98.8, 98.7, 97.4 and <97.0% similarity to RN3S43, LNNU 22110, YIM 93306 and all other current type strains, while strain M4N3S171 had 98.6 and <97.0% similarity to YIM 93306, and all other current type strains, respectively. The average nucleotide identity based on (ANIb) and digital DNA–DNA hybridization (dDDH) values of LR1S40 and M4N3S171 with each other and to the other type strains of were well below the threshold values (95% for ANIb, 70% for dDDH) for differentiating a species. Diphosphatidylglycerol and phosphatidylglycerol were the major polar lipids in both strains. The predominant menaquinone in both strains was both MK-8. The genome of strain LR1S40 consisted of a 3557440 bp circular chromosome, with a G+C content of 71.1 mol%, while the genome of strain M4N3S171 consisted of 4270413 bp, with a G+C content of 67.6 mol%. The phylogenetic, physiological and phenotypic characteristics allowed discrimination of the two strains from their relatives. The names sp. nov. [type strain LR1S40 (=CGMCC 1.19028=KCTC 49726)] and sp. nov. [type strain M4N3S171 (=CGMCC 1. 19142=KCTC 49727)] are therefore proposed. During the publication of , (type strain T3246-1), which was selected as the reference strain for the identification of , was reclassified as . The two phylogenetic trees showed that HY164 tightly clustered with F300, and had the highest 16S rRNA gene similarity (99.8%) to F300. Based on the phylogenetic analysis and the publication record, should be reclassified as comb. nov.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): phylogenetic analysis , Ruania , saline soil and taxonomy
Funding
This study was supported by the:
  • Natural Science Foundation of Inner Mongolia Autonomous Region of China (Award 2021MS03031)
    • Principle Award Recipient: Ji-QuanSun
© 2022 The Authors

Erratum

An erratum has been published for this content:
Corrigendum: sp. nov. and sp. nov., two actinobacteria isolated from saline soil, and reclassification of as comb. nov.
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2022-06-28
2024-04-27
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Ruania suaedae sp. nov. and Ruania halotolerans sp. nov., two actinobacteria isolated from saline soil, and reclassification of Haloactinobacterium kanbiaonis as Occultella kanbiaonis comb. nov.
Int J Syst Evol Microbiol 72, 005443 (2022); https://doi.org/10.1099/ijsem.0.005443
/content/journal/ijsem/10.1099/ijsem.0.005443
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