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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 7

sp. nov., a novel denitrification actinobacterium isolated from microbial fermentation bed material from a pig farm No Access

Abstract

An opaque, pink-coloured, gram-positive, aerobic bacteria (designated as FBM22-1), was isolated from microbial fermentation bed material from a pig farm in northwestern China. Optimal growth occurred at 30–37 °C, pH 7.0 and with 0.5 % NaCl (w/v). The strain had nitrification and denitrification functions. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the novel isolate belonged to the genus . Strain FBM22-1 was closely related to NBRC 100606 and NBRC 16069, with 16S rRNA gene sequence similarities of 97.9 and 97.7 %, respectively. The predominant menaquinone in strain FBM22-1 was MK-8(H). The cellular fatty acids consisted primarily of Cω7 and/or C ω6, C and 10-methyl C. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and glycolipid. The G+C content of strain FBM22-1 was 68.64 mol%. Based on the phenotypic, phylogenetic and chemotaxonomic characterization results, in combination with low values of digital DNA–DNA hybridization between strain FBM22-1 and its closest neighbours, FBM22-1 represents a novel species of the genus , for which the name p. nov. is proposed; the type strain is FBM22-1 (=KCTC 49502=CCTCC AB2020275).

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Keyword(s): denitrification , genome , microbial fermentation bed material , prokaryotic taxonomy and Rhodococcus
© 2022 The Authors
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2022-07-14
2024-04-28
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Rhodococcus yananensis sp. nov., a novel denitrification actinobacterium isolated from microbial fermentation bed material from a pig farm
Int J Syst Evol Microbiol 72, 005426 (2022); https://doi.org/10.1099/ijsem.0.005426
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