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Volume 72, Issue 6

sp. nov., a pathogen causing bacterial rot of parsley in Japan Open Access

Abstract

Phytopathogenic bacterial strains (MAFF 311094, MAFF 311095, MAFF 311096 and MAFF 311097), which were isolated from rot lesions of parsley () sampled in Miyagi, Japan, were subjected to polyphasic characterization to determine their taxonomic position. The cells were Gram-reaction-negative, aerobic, non-spore-forming, motile with one or two polar flagella and rod-shaped. The 16S rRNA gene sequences analyses revealed that the strains belong to the genus , exhibiting the highest sequence similarity to P7 (99.93% sequence similarity), MAFF 301449 (99.93 %), 14-3 (99.86 %), MAFF 212408 (99.86 %) and CMS 35 (99.79 %). The genomic DNA G+C content was 60.1 mol%, and the major cellular fatty acids (>5 % of the total fatty acids) were C, summed feature 3 (C 7/C 6), summed feature 8 (C 7/C 6) and C cyclo. The sequence-based phylogenetic and whole genome-based phylogenomic analyses demonstrated that the strains are a member of the subgroup, but their phylogenetic position does not match those of any members of this subgroup. The average nucleotide identity and digital DNA–DNA hybridization values between the strains and their closely related species were ≤90.64% and ≤41.9 %, respectively, which were below the thresholds for prokaryotic species delineation (95–96 and 70%, respectively). Phenotypic characteristics, pathogenicity toward parsley and cellular fatty acid composition could differentiate the strains from their closest relatives. The phenotypic, chemotaxonomic and genotypic data presented in this study revealed that the strains constitute a novel species, for which we propose the name sp. nov., with MAFF 311094 (=ICMP 24279) being the type strain.

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  • Accepted:
  • Published Online:
Keyword(s): bacterial rot disease , parsley , Petroselinum crispum , phylogenomic analysis , polyphasic taxonomy and Pseudomonas petroselini
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2022-06-13
2024-05-13
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Pseudomonas petroselini sp. nov., a pathogen causing bacterial rot of parsley in Japan
Int J Syst Evol Microbiol 72, 005424 (2022); https://doi.org/10.1099/ijsem.0.005424
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