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Volume 72, Issue 6

sp. nov., an extremely acid-tolerant member of the genus Open Access

Abstract

A nonmotile, facultatively anaerobic and rod-shaped bacterial strain, designated M1 was isolated from a bioreactor being operated at pH ~2 at Brisbane, Australia. Colonies appeared to be convex and white. Phylogenetic analysis of its genome revealed an affiliation with the genus and its closest species based on 16S rRNA gene analysis were DSM 45454 (98.8 % similarity) and CIP 104321 (98.8 %) with which strain M1 shared average nucleotide identity of 81.2 % and digital DNA–DNA hybridization similarity of 23.8 %. Strain M1 grew optimally at 0 % NaCl, at pH 6 and at between 30–33 °C. The polar lipid profile of strain M1 consisted of diphosphatidylglycerol, aminophosphoglycolipid, phosphatidylcholine, phospholipid, aminolipid, phosphoglyolipid, phosphatidylglycerol, two unidentified glycolipids and four unidentified lipids. The dominant cellular fatty acids (>10 %) were C and C 9 and summed feature 7 (C 7 and/or C 6). The DNA G+C content of strain M1 was 69.1 mol%. Based on phylogenomic analysis coupled with physiological and chemotaxonomic characterizations, we classify strain M1 as representing a novel species within the genus , for which the name nov. is proposed. The type strain is M1 (=MCCC 1H00416=KCTC 49392).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): acid-tolerant , Mycolicibacter acidiphilus , phylogenetic analysis and polyphasic analysis
Funding
This study was supported by the:
  • University of Queensland Research Training Scholarship
    • Principle Award Recipient: JunXia
  • Advance Queensland Industry Research Fellowships (Award RM2019002600)
    • Principle Award Recipient: NiGaofeng
  • Australian Research Council (Award DP180103595)
    • Principle Award Recipient: ShihuHu
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2022-06-09
2024-04-29
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Mycolicibacter acidiphilus sp. nov., an extremely acid-tolerant member of the genus Mycolicibacter
Int J Syst Evol Microbiol 72, 005419 (2022); https://doi.org/10.1099/ijsem.0.005419
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