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Volume 72, Issue 6

sp. nov., a novel ligninase-producing Actinobacterium isolated from soil No Access

Abstract

A novel ligninase-producing actinomycete, designated strain NEAU-G4, was isolated from a soil sample and subjected to a polyphasic taxonomic study to establish its status. According to 16S rRNA gene sequence comparisons, the isolate was identified as a member of the genus , with the highest sequence similarity to DSM 44496 (99.2 %). The whole-cell sugars contained galactose and arabinose. The amino acid of the cell wall was determined to be -diaminopimelic acid. The major fatty acids (>10 %) were C, C 9, C and C 7. The predominant menaquinone was identified as MK-8(H, ω-cycl). The major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. Strain NEAU-G4 had a draft genome size of 6 405 167 bp, annotated with 5815 protein-coding genes. The DNA G+C content was 67.6 mol%. Phylogenetic analysis using the 16S rRNA gene and whole-genome sequences showed that strain NEAU-G4 formed a stable phyletic line with DSM 44496. The digital DNA–DNA hybridization and average nucleotide identity values between them were 63.7 % (60.8–66.5 %) and 95.5 %, respectively. Moreover, genomic analysis indicated that strain NEAU-G4 had the potential to degrade lignin and produce bioactive compounds. On the basis of genotypic analysis, physiological data, as well as phenotypic and chemotaxonomic characterizations, it is concluded that the organism be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-G4 (=CCTCC AA 2020038=DSM 111936).

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Keyword(s): 16S rRNA gene sequence , Nocardia rosealba sp. nov. , polyphasic taxonomy and whole-genome analysis
Funding
This study was supported by the:
  • “Academic Backbone” Project of Northeast Agricultural University (Award 19XG18)
    • Principle Award Recipient: JiaSong
  • National Natural Science Foundation of China (Award 31972291)
    • Principle Award Recipient: XiangjingWang
© 2022 The Authors
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2022-06-10
2024-04-26
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Nocardia rosealba sp. nov., a novel ligninase-producing Actinobacterium isolated from soil
Int J Syst Evol Microbiol 72, 005416 (2022); https://doi.org/10.1099/ijsem.0.005416
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