1887

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Volume 72, Issue 6

gen. nov., sp. nov. and gen. nov., sp. nov., two acidobacteria isolated from forest soil, and reclassification of and as gen. nov., comb. nov. and comb. nov. No Access

Abstract

Two aerobic and obligately acidophilic bacteria, designated 4G-K13 and 4Y35, were isolated from the forest soil sampled at Dinghushan Biosphere Reserve, Guangdong Province, PR China. These two strains were Gram-stain-negative, non-motile and short rods that multiplied by binary division. Strains 4G-K13 and 4Y35 had the highest 16S rRNA gene sequence similarity of 97.0 and 97.2 % to DSM 103733 and SBC82, respectively. Phylogenetic trees based on the 16S rRNA gene and whole genome sequences showed consistently that these two strains formed a major clade with members of the genera , , and in the family , but each occupied an unique position. In both the UBCG and the PhyloPhlAn phylogenomic trees, strains 4G-K13 and 4Y35 congruently formed a highly supported subclade with DSM 11244 and DSM 27394, respectively. The major fatty acids (>5 %) of strain 4G-K13 were iso-C, iso-C, summed feature 3 (C 7 and/or C 6) and summed feature 9 (iso-C 9 and/or C 10-methyl), while that of strain 4Y35 were C, C 9, iso-C, summed feature 3 (C 7 and/or C 6) and summed feature 9 (iso-C 9 and/or C 10-methyl). Strain 4G-K13 contained phosphatidylethanolamine, four unidentified phospholipids, four glycolipids, two unidentified aminolipids and two unknown lipids, while strain 4Y35 had phosphatidylethanolamine, three unidentified phospholipids, two glycolipids, five unidentified aminolipids and one unknown polar lipid. The DNA G+C contents of 4G-K13 and 4Y35 were 60.5 and 55.8 mol%, respectively. Based on all these phylogenetic, physiological and chemotaxonomic data, we suggest that strains 4G-K13 and 4Y35 represent two novel species of two novel genera in the family , for which the names gen. nov., sp. nov. (type strain: 4G-K13=GDMCC 1.1195=NBRC 113249) and gen. nov., sp. nov. (type strain: 4Y35=KACC 21728=NBRC 114261) are proposed. We also propose to reclassify and as gen. nov., comb. nov. and comb. nov., respectively, based mainly on the results of phylogenomic analysis.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Acidobacteriaceae , Alloacidobacterium , Paracidobacterium and phylogeny
Funding
This study was supported by the:
  • Guangdong Province Science and Technology Innovation Strategy Special Fund (Award 2018B020205003)
    • Principle Award Recipient: Li-hongQiu
© 2022 The Authors
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2022-06-09
2024-04-28
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Paracidobacterium acidisoli gen. nov., sp. nov. and Alloacidobacterium dinghuense gen. nov., sp. nov., two acidobacteria isolated from forest soil, and reclassification of Acidobacterium ailaaui and Acidipila dinghuensis as Pseudacidobacterium ailaaui gen. nov., comb. nov. and Silvibacterium dinghuense comb. nov.
Int J Syst Evol Microbiol 72, 005415 (2022); https://doi.org/10.1099/ijsem.0.005415
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