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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 6

sp. nov., isolated from the Mariana Trench No Access

Abstract

Two strains, TMB456 and TMB1265, were isolated from different locations in the Mariana Trench. Analysis of the 16S rRNA gene and genomic rRNA sequences indicated that they were from the same novel species and were affiliated with the genus of the class . Phylogenetic analysis based on 16S rRNA gene sequences indicated that the most closely related validly published species were Kr3 (98.1 % similarity) and JAM1 (97.3 % similarity). Digital DNA–DNA hybridization values of TMB456 with Kr3 and JAM1 were <25 %. The average nucleotide identity value between strain TMB456 and Kr3 was 80.9 %. The genomic DNA G+C contents of strains TMB456 and TMB1265 were both 44.9 mol %. Strains TMB456 and TMB1265 could grow at 4–37 °C (optimum at 20–28 °C), at pH 3–10 (optimum at pH 7–9) and in the presence of 0–10 % (w/v) NaCl (optimum at 0–1 %). Cells of strains TMB456 and TMB1265 were Gram-negative rods (0.3–0.6 µm×0.7–1.3 µm). Chemotaxonomic analysis showed that ubiquinone 8 was the sole quinone produced by strain TMB456 and that the major cellular fatty acids were iso-C, summed feature 3 (C 7 and/or C 6) and summed feature 8 (C 7 and/or C 6). The polar lipid profile of this strain included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphoglycolipids and two unidentified polar lipids. Based on the phenotypic, chemotaxonomic and molecular features, strains TMB456 and TMB1265 belong to a novel species within the genus , for which the name sp. nov. is proposed. The type strain is TMB456 (=KCTC 82622= MCCC 1K05898).

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  • Accepted:
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Keyword(s): Mariana Trench , methanol and Methylophaga pinxianii sp. nov.
Funding
This study was supported by the:
  • Science and Technology Research Program of Shanghai (Award 19DZ2282100)
    • Principle Award Recipient: Ting-TingWei
  • National Natural Science Foundation of China (Award 31870109)
    • Principle Award Recipient: Ting-TingWei
  • National Key R&D Program of China (Award 2018YFC0310600)
    • Principle Award Recipient: Zhe-XueQuan
© 2022 The Authors
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Methylophaga pinxianii sp. nov., isolated from the Mariana Trench
Int J Syst Evol Microbiol 72, 005414 (2022); https://doi.org/10.1099/ijsem.0.005414
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