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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 6

sp. nov., isolated from a lava cave No Access

Abstract

A Gram-reaction-positive, strictly aerobic, non-sporulating, non-motile, rod-shaped bacterium, designated YC3-14, was isolated from pieces of stalagmite collected in a lava cave in Jeju, Republic of Korea. Cells showed growth at 15–35 °C, pH 6.0–9.0 and with 0–3 % (w/v) NaCl. Colonies of the cells were circular, smooth, convex and cream in colour. A 16S rRNA gene-based neighbour-joining tree indicated that the organism belonged to the genus and formed a sublineage between an cluster and an cluster. The highest 16S rRNA gene similarity values of strain YC3-14 were with the type strains of (99.2 %), (99.1 %), (98.8 %), (98.8 %) and . (98.7 %). The cell-wall peptidoglycan contained -diaminopimelic acid as the diagnostic diamino acid. The major menaquinone was MK-9(H). The predominant fatty acids were C.10-methyl, C 9 and C. The polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, an unidentified phospholipid and two unidentified lipids. The G+C content of the genome DNA was 69.9 mol%. These chemotaxonomic features of the isolate were typical for the genus . The genome-based phylogeny showed the same tree topology as the 16S rRNA gene phylogeny. The average nucleotide identity (≤84.5 %) and digital DNA–DNA hybridization (≤27.5 %) values supported that the isolate belongs to a novel species of the genus . On the basis of data obtained by a polyphasic approach, strain YC3-14 (=KCTC 49469=NBRC 114653) represents a novel species of the genus , for which the name sp. nov. is proposed.

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Keyword(s): Aeromicrobium , Aeromicrobium stalagmiti sp. nov. , lava cave and popcorns
© 2022 The Authors
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2022-06-08
2024-04-28
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Aeromicrobium stalagmiti sp. nov., isolated from a lava cave
Int J Syst Evol Microbiol 72, 005412 (2022); https://doi.org/10.1099/ijsem.0.005412
/content/journal/ijsem/10.1099/ijsem.0.005412
/content/journal/ijsem/10.1099/ijsem.0.005412
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