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Volume 72, Issue 6

sp. nov., a novel cellulase-producing actinobacterium isolated from soil No Access

Abstract

A novel cellulase-producing actinobacterium, designated strain NEAU-L178, was isolated from soil sample collected from Qiqihaer, Heilongjiang Province, PR China. A polyphasic study was carried out to determine the taxonomic status of the strain. On the basis of 16S rRNA gene sequence analysis, strain NEAU-L178 should be classified into the genus and is closely related to SYSU K10005 (99.31 % 16S rRNA gene sequence similarity), NEAU-BB2C19 (98.75 %), NEAU-ZJ3 (98.75 %) and ‘’ NEAU-mq18 (98.34 %). The digital DNA–DNA hybridization values between them are 27.1, 26.1, 42.0 and 30.9 %, and the whole-genome average nucleotide identity values between them are 83.1, 82.3, 90.3 and 85.8 %, respectively. The whole-cell hydrolysates contained glucose, ribose, arabinose and madurose. The menaquinones were identified as MK-9(H), MK-9(H) and MK-9(H). The major fatty acids were C, iso-C and C 10-methyl. The detected polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, phosphatidylinositol and three unidentified phospholipids. The genomic DNA G+C content was 69.7 mol%. In addition, whole-genome analysis indicated that strain NEAU-L178 had the potential to degrade cellulose. Based on the phenotypic, genotypic, chemotaxonomic and phylogenetic data, strain NEAU-L178 can be differentiated from its close phylogenetic relatives and represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NEAU-L178 (=JCM 34799=CGMCC 4.7741).

  • Received:
  • Accepted:
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Keyword(s): 16S rRNA gene , cellulase-producing actinobacterium , genome , Nonomuraea aurantiaca sp. nov. and polyphasic approach
Funding
This study was supported by the:
  • the National Natural Science Foundation of China (Award 31972291)
    • Principle Award Recipient: XiangjingWang
© 2022 The Authors
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2022-06-06
2024-04-26
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Nonomuraea aurantiaca sp. nov., a novel cellulase-producing actinobacterium isolated from soil
Int J Syst Evol Microbiol 72, 005411 (2022); https://doi.org/10.1099/ijsem.0.005411
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