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Abstract
Three Gram-stain-positive bacterial strains (designated F502-1T, F575-4T and F582-1) were isolated from the gut of honeybee (Apis mellifera). These strains were characterized using a polyphasic taxonomic approach. Results of 16S rRNA gene sequence analysis indicated that strain F502-1T was phylogenetically related to the type strains of Apilactobacillus kunkeei , Apilactobacillus nanyangensis and Apilactobacillus apinorum , having 98.7–99.9 % 16S rRNA gene sequence similarities, 84.6–92.5 % pheS sequence similarities and 95.2–99.0 % rpoA sequence similarities, and that strains F575-4T and F582-1 were closely related to the type strain of Apilactobacillus ozensis , having 99.7 and 99.4 % 16S rRNA gene sequence similarities, respectively. Strains F575-4T and F582-1 had less than 88.7 % pheS and 96.4 % rpoA sequence similarities to strain F502-1T and type strains of all Apilactobacillus species. The average nucleotide identity and digital DNA–DNA hybridization values between strains F502-1T, F575-4T, F582-1 and type strains of all Apilactobacillus species were less than 91.3 and 43.5 %, respectively, confirming that they represent two novel species within the genus Apilactobacillus . Based upon the data obtained in the present study, two novel species, Apilactobacillus zhangqiuensis sp. nov. and Apilactobacillus xinyiensis sp. nov., are proposed and the type strains are F502-1T (=JCM 34500T=CCTCC AB 2021026T) and F575-4T (=JCM 34501T=CCTCC AB 2021028T), respectively.
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Funding
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National Natural Science Foundation of China
(Award no. 31471594)
- Principle Award Recipient: ChunTao Gu