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Volume 72, Issue 5

sp. nov. and sp. nov., isolated from landfill leachate of a domestic waste treatment plant Open Access

Abstract

Two strains designated as c1 and c7, were isolated from the landfill leachate of a domestic waste treatment plant in Huizhou City, Guangdong Province, PR China. The cells of both strains were aerobic, rod-shaped, non-motile and formed yellow colonies on Reasoner’s 2A agar plates. Strain c1 grew at 10–42 °C (optimum, 30 °C), pH 4.5–10.5 (optimum, pH 7.0) and 0–2.0 % (w/v) NaCl (optimum, 0–0.5 %). Strain c7 grew at 10–42 °C (optimum, 30 °C), pH 4.5–10.5 (optimum, pH 6.0) and 0–2.0 % (w/v) NaCl (optimum, 0–0.5 %). Phylogenetic analyses revealed that strains c1 and c7 belong to the genus . The 16S rRNA gene sequence similarities of strains c1 and c7 to the type strains of species were 94.5–98.2 % and 94.3–99.1 %, respectively. The calculated pairwise average nucleotide identity values among strains c1, c7 and the reference strains were in the range of 75.2–85.9 % and the calculated pairwise average amino acid identity values among strains c1, c7 and reference strains were in the range of 72.0–88.3 %. Their major respiratory quinone was Q-10, and the major cellular fatty acids were C 7, C, C 7, C and C 2OH. The major polar lipids of strains c1 and c7 were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, sphingoglycolipid, unidentified lipids and unidentified phospholipid. Based on phenotypic, chemotaxonomic, phylogenetic and genomic results from this study, strains c1 and c7 should represent two independent novel species of , for which the names sp. nov. (type strain c1=GDMCC 1.2555=KCTC 82826) and sp. nov. (type strain c7=GDMCC 1.2556=KCTC 82827) are proposed. The gene function annotation results of strains c1 and c7 suggest that they could play an important role in the degradation of organic pollutants.

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  • Accepted:
  • Published Online:
Keyword(s): identification , landfill leachate , new species and Novosphingobium
Funding
This study was supported by the:
  • GDAS’ Special Project of Science and Technology Development (Award 2022GDASZH-2022010105; 2021GDASYL-20210302001)
    • Principle Award Recipient: MeiyingXu
  • Hubei Technological Innovation Special Fund (Award 2018B020205003; 2019B110205003)
    • Principle Award Recipient: MeiyingXu
  • National Key Research and Development Program of China (Award 2021YFA0910300)
    • Principle Award Recipient: MeiyingXu
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2022-05-27
2024-04-26
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Novosphingobium percolationis sp. nov. and Novosphingobium huizhouense sp. nov., isolated from landfill leachate of a domestic waste treatment plant
Int J Syst Evol Microbiol 72, 005394 (2022); https://doi.org/10.1099/ijsem.0.005394
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