1887

Abstract

A Gram-stain-negative, aerobic, non-motile, rod-shaped or occasionally filamentous-shaped bacterial strain, designated 5GH13-10, was isolated from greenhouse soil sampled in Yeoju-si, Republic of Korea. Colonies were milky-coloured, round and convex, and catalase- and oxidase-positive. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 5GH13-10 was related to the genus and had highest 16S rRNA gene sequence identity with Vu-144 (98.4 %). The major cellular fatty acids were iso-C, iso-C G, iso-C 3-OH and summed feature 3 (C 6 and/or C 7). The predominant quinone was menaquinone MK-7, and the polar lipids were composed of phosphatidylethanolamine, one unidentified aminolipid, three unidentified aminophospholipids, one unidentified phospholipid and five unidentified lipids. The genomic DNA G+C content of strain 5GH13-10 was 43.8 mol%. The average nucleotide identity values between strain 5GH13-10 and the closely related species Gsoil 809, Vu-144 and KIS59-12 were 74.86, 74.74 and 69.52 %, and the digital DNA-DNA hybridization values were 20.0, 19.8 and 18.6 %, respectively. Combined phenotypic, phylogenetic and genomic data demonstrated that strain 5GH13-10 is representative of a novel species of the genus , for which we propose the name sp. nov. (type strain 5GH13-10=KACC 18014=NBRC 113162).

Funding
This study was supported by the:
  • National Institute of Agricultural Sciences, Rural Development Administration (Award PJ013549)
    • Principle Award Recipient: Soon-WoKwon
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/content/journal/ijsem/10.1099/ijsem.0.005391
2022-05-27
2022-07-02
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