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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 5

sp. nov., a methylotrophic methanogen isolated from sediment of the East China Sea No Access

Abstract

A novel methylotrophic methanogen sp. nov. was isolated from East China Sea sediment. Type strain LMO-1 of sp. nov. was irregular 1–2 µm cocci without flagella. Strain LMO-1 could utilize a variety of methylated compounds including methanol, methylamine, dimethylamine and trimethylamine for growth and methanogenesis, while H/CO or acetate could not be used for growth or methanogenesis. Optimum growth temperature was 30–35 °C, optimum pH range for growth was 7.0–7.5, while the optimum salinity spectrum for growth was 1.0%–5.0% NaCl. Based on 16S rRNA gene similarity, strain LMO-1 belongs to , with the highest sequence similarity to DSM 2657 (99.8 %), SLH33(99.4 %), followed by AK-5(98.1 %), DSM 6242 (98.0 %). Digital DNA–DNA hybridization also showed highest similarity with DSM 2657, with the value of 58.4 %. The average nucleotide identity between strain LMO-1 and DSM 2657 was 94.06 %. In summary, LMO-1 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LMO-1 (=MCCC 4K00106=JCM 39195).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): anaerobic , Archaea , marine sediment , methanogen , methanogenesis and methylotrophic
Funding
This study was supported by the:
  • Natural Science Foundation of China (Award 41921006)
    • Principle Award Recipient: FengpingWang
  • COMRA project (Award DY135-B2-12)
    • Principle Award Recipient: FengpingWang
  • National Key Research and Development Project of China (Award 2018YFC0310803)
    • Principle Award Recipient: FengpingWang
© 2022 The Authors
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2022-05-18
2024-05-10
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Methanococcoides orientis sp. nov., a methylotrophic methanogen isolated from sediment of the East China Sea
Int J Syst Evol Microbiol 72, 005384 (2022); https://doi.org/10.1099/ijsem.0.005384
/content/journal/ijsem/10.1099/ijsem.0.005384
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