1887

Abstract

Two lactic acid bacterial strains (IMAU80584 and IMAU92037) were isolated from naturally fermented dairy products (kurut and yoghurt) in China and Russia. Based on sequence analysis of the 16S rRNA gene it was revealed that these strains belonged to . However, phylogenetic tree analyses of two housekeeping genes, (encoding RNA polymerase alpha subunit) and (encoding phenylalanyl-tRNA synthase alpha subunit), and 88 core genes, indicated the two strains were separated into an independent monophyletic branch from DSM 19907, forming an infra-specific subgroup. The average nucleotide identity and digital DNA–DNA hybridization values between IMAU80584 and DSM 19907 were 93.1 and 52.8 %, respectively. Strains IMAU80584 and IMAU92037 are distinguished from DSM 19907 because they have different polar lipids and fatty acids. The novel subgroup strains could not ferment gluconate potassium. The DNA G+C content of strain IMAU80584 was 42.3 mol%. The major cellular fatty acids were C, C 9t and summed feature 5 (C ante and/or C 6 and/or C 9c). Therefore, based on the results of polyphasic taxonomic analysis, IMAU80584 and IMAU92037 could be considered as a novel subspecies in the species with the proposed name subsp. subsp. nov. The type strain is IMAU80584 (=GDMCC 1.2566=JCM 34647).

Funding
This study was supported by the:
  • Inner Mongolia Autonomous Region Science and Technology Project (Award 2021GG0080)
    • Principle Award Recipient: NotApplicable
  • Major projects of Natural Science Foundation of Inner Mongolia Autonomous Region (Award 2019ZD06)
    • Principle Award Recipient: NotApplicable
  • National Natural Science Foundation of China (Award No. 31972095)
    • Principle Award Recipient: NotApplicable
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/content/journal/ijsem/10.1099/ijsem.0.005359
2022-05-06
2024-06-16
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