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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 5

sp. nov., a moderately halophilic bacterium isolated from a soda lake No Access

Abstract

A Gram-stain-positive, aerobic, endospore-forming and rod-shaped bacterium (KQ-3), which grew at 10–45 °C (optimum 35 °C), pH 8.0–10.5 (optimum pH 9.0) and in the presence of 0–16 % (w/v) NaCl (optimum 3.0 %), was isolated from a soda lake and identified as representing a novel species using a polyphasic taxonomic approach. Strain KQ-3 was catalase-positive, oxidase-negative and non-motile. Phylogenetic analysis based on 16S rRNA gene sequence affiliated KQ-3 to the genus and showed the highest similarities to M30 (97.90 %), K1-5 (97.84 %) and FJAT-45347 (97.22 %). Digital DNA–DNA hybridization and average nucleotide identity analyses revealed that KQ-3 displayed 21.4 and 72.81% genomic DNA relatedness with the most closely related strain, M30, respectively. KQ-3 contained all of the conserved signature indels that are specific for members of the genus . The DNA G+C content was 45.03 mol%. The cell-wall peptidoglycan contained -diaminopimelic acid and the polar lipids consisted of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and one unidentified phospholipid. The predominant menaquinone was MK-7 (100%) and the major fatty acids (>10 %) comprised anteiso-C, iso-C and iso-C. Based on the data from the current polyphasic studies, KQ-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KQ-3 (=ACCC 61799=KCTC 33933).

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Keyword(s): alkaliphile , Alteribacter , Alteribacter keqinensis sp. nov. , conserved signature indel , moderately halophilic and soda lake
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41877061)
    • Principle Award Recipient: YananWang
  • Fundamental Research Funds for Central Non-profit Scientific Institution (Award 1610042018005)
    • Principle Award Recipient: HaishengWang
© 2022 The Authors
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2022-05-03
2024-05-02
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Alteribacter keqinensis sp. nov., a moderately halophilic bacterium isolated from a soda lake
Int J Syst Evol Microbiol 72, 005351 (2022); https://doi.org/10.1099/ijsem.0.005351
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