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Volume 72, Issue 5

sp. nov., isolated from the cloaca of a giant Asian pond turtle () No Access

Abstract

A bacterial strain designated 27C isolated from the cloaca of a giant Asian pond turtle was subjected to polyphasic taxonomic characterization. The strain was Gram-stain negative and oxidase- and catalase-positive. It had highest 16S rRNA gene sequence similarity to GCS-AN-3 (97.6 %) and GY511 96.0% and less than 96.0 % to other established species including YS3.2.7, SK3863, E-24608 and DSM 14619. Phylogenetically, strain 27C formed a branch with GCS-AN-3 within the clade. The genome size was 4.32 Mbp and the G+C content was 65.7 mol%. Strain 27C shared highest ANIb values with GCS-AN-3 (82.71/82.73 %) followed by KADR8-3 (78.9/79.0 %) and BD-1 (73.3/75.2 %). The diagnostic diamino acid of the peptidoglycan was diaminopimelic acid and the quinone system was ubiquinone Q-8. Predominant compounds in the polar lipid profile were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine. Major polyamines were 2-hydroxyputrescine and putrescine. In the fatty acid profile, summed feature 3 (C 7 and/or C 6), C, summed feature 8 (C 7 and/or C 6), C, C 3-OH and C 2-OH were detected. All these data identify strain 27C as representing a novel species of the genus and hence we propose the name sp. nov. The type strain is 27C (=CCM 9138=LMG 32213).

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Keyword(s): chemotaxonomy , genome , Ottowia testudinis , phylogeny and physiology
© 2022 The Authors
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2022-05-23
2024-05-06
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Ottowia testudinis sp. nov., isolated from the cloaca of a giant Asian pond turtle (Heosemys grandis)
Int J Syst Evol Microbiol 72, 005348 (2022); https://doi.org/10.1099/ijsem.0.005348
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