1887

Abstract

A bacterial strain designated 27C isolated from the cloaca of a giant Asian pond turtle was subjected to polyphasic taxonomic characterization. The strain was Gram-stain negative and oxidase- and catalase-positive. It had highest 16S rRNA gene sequence similarity to GCS-AN-3 (97.6 %) and GY511 96.0% and less than 96.0 % to other established species including YS3.2.7, SK3863, E-24608 and DSM 14619. Phylogenetically, strain 27C formed a branch with GCS-AN-3 within the clade. The genome size was 4.32 Mbp and the G+C content was 65.7 mol%. Strain 27C shared highest ANIb values with GCS-AN-3 (82.71/82.73 %) followed by KADR8-3 (78.9/79.0 %) and BD-1 (73.3/75.2 %). The diagnostic diamino acid of the peptidoglycan was diaminopimelic acid and the quinone system was ubiquinone Q-8. Predominant compounds in the polar lipid profile were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylmonomethylethanolamine. Major polyamines were 2-hydroxyputrescine and putrescine. In the fatty acid profile, summed feature 3 (C 7 and/or C 6), C, summed feature 8 (C 7 and/or C 6), C, C 3-OH and C 2-OH were detected. All these data identify strain 27C as representing a novel species of the genus and hence we propose the name sp. nov. The type strain is 27C (=CCM 9138=LMG 32213).

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2022-05-23
2022-07-06
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References

  1. Spring S, Jäckel U, Wagner M, Kämpfer P. Ottowia thiooxydans gen. nov., sp nov., a novel facultatively anaerobic, N2O-producing bacterium isolated from activated sludge, and transfer of Aquaspirillum gracile to Hylemonella gracilis gen. nov., comb. nov. Int J Syst Evol Microbiol 2004; 54:99–106 [View Article] [PubMed]
    [Google Scholar]
  2. Cao J, Lai Q, Liu Y, Li G, Shao Z. Ottowia beijingensis sp. nov., isolated from coking wastewater activated sludge, and emended description of the genus Ottowia. Int J Syst Evol Microbiol 2014; 64:963–967 [View Article] [PubMed]
    [Google Scholar]
  3. Pang F-H, Yang H-Y, Sun J, Yu X, Zhang H. Ottowia caeni sp. nov., a novel phenylacetic acid degrading bacterium isolated from sludge. Int J Syst Evol Microbiol 2021; 71:5144–5151 [View Article] [PubMed]
    [Google Scholar]
  4. Shi S-B, Li G-D, Yang L-F, Liu C, Jiang M-G et al. Ottowia flava sp. nov.isolated from fish intestines. Antonie van Leeuwenhoek 2015; 107:1437–1444 [View Article]
    [Google Scholar]
  5. Yi KJ, Im WT, Kim DW, Kim SK. Ottowia konkukae sp. nov., isolated from rotten biji (tofu residue). Int J Syst Evol Microbiol 2018; 68:3458–3462 [View Article] [PubMed]
    [Google Scholar]
  6. Heo J, Cho H, Hong S-B, Kim J-S, Kwon S-W et al. Ottowia oryzae sp. nov., isolated from Andong sikhye, a Korean traditional rice beverage. Int J Syst Evol Microbiol 2018; 68:3096–3100 [View Article] [PubMed]
    [Google Scholar]
  7. Felföldi T, Kéki Z, Sipos R, Márialigeti K, Tindall BJ et al. Ottowia pentelensis sp. nov., a floc-forming betaproteobacterium isolated from an activated sludge system treating coke plant effluent. Int J Syst Evol Microbiol 2011; 61:2146–2150 [View Article] [PubMed]
    [Google Scholar]
  8. Geng S, Pan X-C, Mei R, Wang Y-N, Sun J-Q et al. Ottowia shaoguanensis sp. nov., isolated from coking wastewater. Curr Microbiol 2014; 68:324–329 [View Article]
    [Google Scholar]
  9. Moaledj K. Comparison of Gram-staining and alternate methods, KOH test and aminopeptidase activity in aquatic bacteria: their application to numerical taxonomy. J Microbiol Methods 1986; 5:303–310 [View Article]
    [Google Scholar]
  10. Kämpfer P, Steiof M, Dott W. Microbiological characterisation of a fuel-oil contaminated site including numerical identification of heterotrophic water and soil bacteria. Microb Ecol 1991; 21:227–251 [View Article] [PubMed]
    [Google Scholar]
  11. Lane DJ. 16S/23S rRNA sequencing. In Stackebrandt E, Goodfellow M. eds Nucleic Acid Techniques in Bacterial Systematics Chichester: Wiley; 1991 pp 115–175
    [Google Scholar]
  12. Yoon SH, Ha SM, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: A taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article] [PubMed]
    [Google Scholar]
  13. Felsenstein J. PHYLIP (Phylogeny Inference Package) Version 3.695 Seattle, WA: Department of Genome Sciences, University of Washington; 2013
    [Google Scholar]
  14. Hennig-Pauka I, Sudendey C, Kleinschmidt S, Ruppitsch W, Loncaric I et al. Swine conjunctivitis associated with a novel mycoplasma species closely related to Mycoplasma hyorhinis. Pathogens 2020; 10:13 [View Article] [PubMed]
    [Google Scholar]
  15. Wick RR, Judd LM, Gorrie CL, Holt KE. Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads. PLoS Comput Biol 2017; 13:e1005595 [View Article] [PubMed]
    [Google Scholar]
  16. Carattoli A, Zankari E, García-Fernández A, Voldby Larsen M, Lund O et al. In silico detection and typing of plasmids using PlasmidFinder and plasmid multilocus sequence typing. Antimicrob Agents Chemother 2014; 58:3895–3903 [View Article] [PubMed]
    [Google Scholar]
  17. Richter M, Rosselló-Móra R, Oliver Glöckner F, Peplies J. JSpeciesWS: a web server for prokaryotic species circumscription based on pairwise genome comparison. Bioinformatics 2016; 32:929–931 [View Article] [PubMed]
    [Google Scholar]
  18. Wattam AR, Gabbard JL, Shukla M, Sobral BW. Comparative genomic analysis at the PATRIC, a bioinformatic resource center. Methods Mol Biol 2014; 1197:287–308 [View Article] [PubMed]
    [Google Scholar]
  19. Stamatakis A. RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 2014; 30:1312–1313 [View Article] [PubMed]
    [Google Scholar]
  20. Stamatakis A, Hoover P, Rougemont J. A rapid bootstrap algorithm for the RAxML Web servers. Syst Biol 2008; 57:758–771 [View Article] [PubMed]
    [Google Scholar]
  21. Schumann P. Peptidoglycan structure. Methods Microbiol 2011; 38:101–129
    [Google Scholar]
  22. Tindall BJ. Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 1990; 66:199–202
    [Google Scholar]
  23. Tindall BJ. A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. Syst Appl Microbiol 1990; 13:128–130 [View Article]
    [Google Scholar]
  24. Altenburger P, Kämpfer P, Makristathis A, Lubitz W, Busse H-J. Classification of bacteria isolated from a medieval wall painting. J Biotechnol 1996; 47:39–52 [View Article]
    [Google Scholar]
  25. Stolz A, Busse HJ, Kämpfer P. Pseudomonas knackmussii sp. nov. Int J Syst Evol Microbiol 2007; 57:572–576 [View Article] [PubMed]
    [Google Scholar]
  26. Yan Z-F, Trinh H, Moya G, Lin P, Li C-T et al. Ramlibacter rhizophilus sp. nov., isolated from rhizosphere soil of national flower Mugunghwa from South Korea. Int J Syst Evol Microbiol 2017; 67:3773–3777 [View Article] [PubMed]
    [Google Scholar]
  27. Kämpfer P, Kroppenstedt RM. Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 1996; 42:989–1005 [View Article]
    [Google Scholar]
  28. Busse J, Auling G. Polyamine pattern as a chemotaxonomic marker within the Proteobacteria. Syst Appl Microbiol 1988; 11:1–8 [View Article]
    [Google Scholar]
  29. Busse H-J, Bunka S, Hensel A, Lubitz W. Discrimination of members of the family Pasteurellaceae based on polyamine patterns. Int J Syst Evol Microbiol 1997; 47:698–708 [View Article]
    [Google Scholar]
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