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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 72, Issue 3

sp. nov., a novel endophytic bacterium isolated from bark of No Access

Abstract

A new endophytic bacterium, designated strain MQZ13P-4 was isolated from collected from Maowei sea Mangrove Nature Reserve in Guangxi Zhuang Autonomous Region, PR China. The 16S rRNA gene sequence similarity between strain MQZ13P-4 and its closest phylogenetic neighbour CBS5Q-3 was 97.9 %. Phylogenetic analyses using 16S rRNA gene sequences and whole-genome sequences showed that strain MQZ13P-4 formed a distinct lineage with CBS5Q-3, 40Bstr34 and JCM 30119. The draft genome of strain MQZ13P-4 was 5 153 243 bp in size and its DNA G+C content was 68.1 mol%. Comparative genome analysis revealed that the average nucleotide identity, digital DNA–DNA hybridization and average amino acid identity values among strain MQZ13P-4 and other related species were below the cut-off levels of 95, 70 and 95.5 %, respectively. The cell-wall peptidoglycan of strain MQZ13P-4 contained -diaminopimelic acid as the diagnostic diamino acid. The respiratory quinone was Q-10. The major cellular fatty acid was C 7. The polar lipids comprised phosphatidylcholine, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two unidentified aminolipids and two unidentified lipids. Strain MQZ13P-4 had a typical chemical compositions of fatty acids, lipids, quinones and diagnostic diamino acid for species, but could be distinguished from known species of the genus . Based on polyphasic evidence, strain MQZ13P-4 represents novel species of the genus , for which the name sp. nov. is proposed. The type strain is MQZ13P-4 (=CGMCC 1.18727=JCM 34333).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genomics , Genus Jiella , Jiella sonneratiae and polyphasic taxonomy
Funding
This study was supported by the:
  • Zunyi Science and Technology Plan Project (Award [2019]19)
    • Principle Award Recipient: Hai-BoYi
  • Science and Technology Fund Project of Guizhou Provincial Health Commission (Award gzwjkj2019-1-042)
    • Principle Award Recipient: Hai-BoYi
  • Guizhou Provincial Science and Technology Foundation (Award Qian Ke He Jichu [2019]1347)
    • Principle Award Recipient: LiTuo
  • National Natural Science Foundation of China (Award 81960642)
    • Principle Award Recipient: LiTuo
© 2022 The Authors
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2022-03-30
2025-01-24
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Jiella sonneratiae sp. nov., a novel endophytic bacterium isolated from bark of Sonneratia apetala
Int J Syst Evol Microbiol 72, 005310 (2022); https://doi.org/10.1099/ijsem.0.005310
/content/journal/ijsem/10.1099/ijsem.0.005310
/content/journal/ijsem/10.1099/ijsem.0.005310
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