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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 3

sp. nov., a carbon monoxide-oxidizing acetogen isolated from old hay No Access

Abstract

An acid/alcohol-producing, Gram-stain-positive, obligately anaerobic, rod-shaped, non-motile, non-spore forming acetogen, designated as strain P21, was isolated from old hay after enrichment with CO as the substrate. Spores not observed even after prolonged incubation (30 days). Phylogenetic analysis of the 16S rRNA gene sequence of strain P21 showed it was closely related to DSM 15243 (97.9%), DSM 757 (97.7 %) and DSM 12750 (97.7 %). The genome is 5.6 Mb and the G+C content is 29.4 mol%. Average nucleotide identity between strain P21, , and was 87.1, 86.4, 86.4 %, respectively. Strain P21 grew on CO:CO, H:CO, -arabinose, ribose, xylose, fructose, galactose, glucose, lactose, mannose, cellobiose, sucrose, cellulose, starch, pyruvate, choline, glutamate, histidine, serine, threonine and casamino acids. End products of metabolism were acetate, butyrate, caproate, ethanol and hexanol. Dominant cellular fatty acids (>10 %) were C (41.5 %), C ω7/C ω6 (10.0 %), and a summed feature containing cyclo C/C (17.3 %). Based on the phenotypic, chemotaxonomic, phylogenetic and phylogenomic analyses, strain P21 represents a new species in the genus , for which the name sp. nov. is proposed. The type strain is P21 (=DSM 111390=NCIMB 15261).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): acetogens , Clostridium and CO-oxidizing
Funding
This study was supported by the:
  • Cooperative State Research, Education, and Extension Service (Award 01-34447-10302)
    • Principle Award Recipient: RalphS. Tanner
© 2022 The Authors
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2022-03-30
2024-05-01
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Clostridium muellerianum sp. nov., a carbon monoxide-oxidizing acetogen isolated from old hay
Int J Syst Evol Microbiol 72, 005297 (2022); https://doi.org/10.1099/ijsem.0.005297
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