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Abstract

A Gram-stain-positive bacterial strain, designated G127AT, was isolated as soft small white colonies from the hindgut of the cockroach . Examination of the complete 16S rRNA sequence mapped the strain to the genus . The type strain with the highest pairwise similarity was H23-8 (97.3%). The genome of G127AT was sequenced by a combination of Illumina and Nanopore methods and consisted of a single circular DNA molecule with a size of 3.45 Mb. The DNA G+C content was 71.3 mol%. A phylogenomic tree based on conserved single copy housekeeping genes, placed G127AT among the ancestral species of the genus , and only P27 was found to diverge earlier than G127AT. Genome distance metrics average nucleotide identity (ANI) (76–78 %) and digital DNA–DNA hybridization (dDDH) (20.2–21.5 %) of the isolate against available genomes of several type strains of species of the genus indicated that G127AT represented a previously undescribed species of the genus . Morphological, physiological and biochemical characteristics, including lipid profile, cellular fatty acids and peptidoglycan type were in accordance with usual attributes of members of the genus . The novel isolate could be differentiated from the most closely related species by extracellular expression of acid and alkaline phosphatases, trypsin and α-chymotrypsin, and utilization of -arabinose and salicin as sole carbon sources. On the basis of the combined genomic and phenotypic features, isolate G127AT (=DSM 111850=LMG 32099) is considered to represent a novel species of the genus , for which we propose the name sp. nov.

Funding
This study was supported by the:
  • Ministerio de Ciencia, Innovación y Universidades (Award PGC2018-096185-B-I00)
    • Principle Award Recipient: MarthaTrujillo
  • LOEWE Zentrum AdRIA
    • Principle Award Recipient: AndreasVilcinskas
  • Alexander von Humboldt-Stiftung
    • Principle Award Recipient: JuanDavid Guzman
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/content/journal/ijsem/10.1099/ijsem.0.005283
2022-03-21
2024-04-17
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