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Volume 72, Issue 3

sp. nov., a novel polyhydroxyalkanoate-accumulating bacterium Open Access

Abstract

A Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacterium, capable of producing poly-β-hydroxyalkanoate, designated DP3N28-2, was isolated from the sediment collected from Daya Bay, Guangdong, PR China. Optimal growth occurred at 37–40 °C, pH 6.0 and in the presence of 4 % NaCl. The 16S rRNA gene sequences analysis revealed that DP3N28-2 showed highest similarities with DSM 23384 (98.3 %), 13–2-B6 (97.2 %), s El-219 (96.8 %), MM-10 (96.7 %), CL-GR66 (96.4 %) and L1 8-17 (96.1 %). The predominant fatty acids (>10 %) were summed feature 8 (Cω6 and/or Cω7; 72.1 %) and C (11.0 %). The polar lipids contain phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol, one aminophosphlipid, one phospholipid and three unidentified lipids. The respiratory quinone was Q-10. The DNA G+C content was 63.0 mol% (data from the genome sequence). The estimated genome size was 5.12 Mb. The average nucleotide identity values between the DP3N28-2 genome and the genome of was 81.1 %, while the digital DNA–DNA hybridization value was 23.4 %. The phenotypic, genotypic and chemotaxonomic differences between DP3N28-2 and its phylogenetic relatives indicates that DP3N28-2 should be regarded as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DP3N28-2 (=MCCC 1K06218=KCTC 82804).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): ANI , dDDH , draft genome sequencing , Mameliella and polyhydroxyalkanoate
© 2022 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2022-03-09
2024-05-12
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Mameliella sediminis sp. nov., a novel polyhydroxyalkanoate-accumulating bacterium
Int J Syst Evol Microbiol 72, 005274 (2022); https://doi.org/10.1099/ijsem.0.005274
/content/journal/ijsem/10.1099/ijsem.0.005274
/content/journal/ijsem/10.1099/ijsem.0.005274
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