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Volume 72, Issue 3

sp. nov., a carotenoid-producing bacterium isolated from surface seawater No Access

Abstract

A Gram-negative, moderately halophilic bacterium, designated as strain Y3S6, was isolated from a surface seawater sample collected from Dongangyoeng cave, Udo-myeon, Jeju-si, Jeju-do, Repulic of Korea. Cells of strain Y3S6 were aerobic, rod-shaped, non-sporulated, yellow, catalase- negative, oxidase-negative and motile with one polar flagellum. Growth of strain Y3S6 occurred at 15–40 °C (optimum: 25–30 °C), at pH 6.0–9.0 (optimum: pH 7.0) and in the presence of 0–13% NaCl (optimum: 1–6 %, w/v). The novel strain was able to produce carotenoids. Its chemotaxonomic and morphological characteristics were consistent with those of members of the genus . Phylogenetic analysis of the 16S rRNA gene sequence revealed that strain Y3S6 formed a clade with L5 (98.97 %) and LCB169(98.90%). The average nucleotide identity and digital DNA–DNA hybridization values of strain Y3S6 with the most closely related strains for which whole genomes are publicly available were 82.3–85.2% and 62.8–66.1 %, respectively. The major fatty acids in strain Y3S6 were C, C cyclo ω8 and summed feature 8 (composed of C ω7 and/or C ω6), and the predominant quinone was Q-9. Its polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phosphoglycolipid, one unidentified phosphoaminoglycolipid and one unidentified phospholipid. The genomic DNA G+C content based on the draft genome sequence was 64.2 mol%. The results of physiological and biochemical tests and 16S rRNA sequence analysis clearly revealed that strain Y3S6 represents a novel species in the genus , for which the name sp. nov. has been proposed. The type strain is Y3S6 (=KACC 21536=NBRC 114315=TBRC 15164).

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Keyword(s): carotenoid , Halomonadaceae , Halomonas antri , moderately halophile , moderately halophilic bacteria and seawater
© 2022 The Authors
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2022-03-03
2024-05-01
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Halomonas antri sp. nov., a carotenoid-producing bacterium isolated from surface seawater
Int J Syst Evol Microbiol 72, 005272 (2022); https://doi.org/10.1099/ijsem.0.005272
/content/journal/ijsem/10.1099/ijsem.0.005272
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