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Volume 72, Issue 7

sp. nov., isolated from rhizosphere of No Access

Abstract

An actinobacterial strain, designated R-N-C8, was isolated from the rhizosphere soil of collected in Yunnan Province, south-west China. Based on the results of 16S rRNA gene sequence analysis, strain R-N-C8 had highest similarity to CGMCC 1.7056 (96.5%), KCTC 19804 (96.3%) and IB-3 (96.1%), and lower than 96.0 % similarity to other members of the genus . Phylogenetic trees based on 16S rRNA gene sequences indicated that strain R-N-C8 formed an isolated branch with CGMCC 1.7056 and KCTC 19804. The polar lipids contained phosphatidylglycerol, diphosphatidylglycerol, one unidentified phosphoglycolipid and four unidentified phospholipids in the cellular membrane. The major fatty acids were identified as iso-C, anteiso-C, iso-C, summed feature 9 (iso-C ω9 and/or C 10-methyl) and iso-C. The predominant respiratory quinone was MK-8(H) and -diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The genomic DNA G+C content was 70.9 mol%. The orthologous average nucleotide identiy values between CGMCC 1.7056, IB-3 and strain R-N-C8 were 77.1 and 75.1 %, respectively. DNA–DNA hybridization values between CGMCC 1.7056, IB-3 and strain R-N-C8 were 20.7 and 19.9 % respectively. Data from phenotypic and genotypic analyses supported that strain R-N-C8 represents a new species of , for which the name sp. nov. is proposed. The type strain is R-N-C8 (=CGMCC 1.18723= KCTC 49528).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Arabidopsis thaliana , Nocardioides nematodiphilus sp. nov. , polyphasic taxonomy and rhizosphere
Funding
This study was supported by the:
  • Science Foundation of China (Award 31870091)
    • Principle Award Recipient: NotApplicable
  • Tobacco Company of Yunnan (Award 2021530000241006)
    • Principle Award Recipient: NotApplicable
© 2022 Yunnan University P.R. China
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2022-07-14
2024-04-29
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Nocardioides nematodiphilus sp. nov., isolated from rhizosphere of Arabidopsis thaliana
Int J Syst Evol Microbiol 72, 005271 (2022); https://doi.org/10.1099/ijsem.0.005271
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