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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 3

sp. nov., isolated from rhizomes No Access

Abstract

Through bacterial plant–endophyte extraction from rhizomes of plant, a Gram-stain-negative, aerobic, catalase- and oxidase-positive gammaproteobacterial strain, referred to as FIT28, was isolated. FIT28 shows vigorous growth on nutrient rich media within the temperature range of 4–35 °C, with optimal growth at 28 °C, a wide pH tolerance from pH 5 to 11, and salt tolerance up to 6 % (w/v) NaCl. Colonies are white-yellow and quickly become mucoid. The results of analysis of the 16S rRNA gene sequence placed the strain within the genus , and multilocus sequence analysis (MLSA) using 16S rRNA, , and concatenated sequences revealed that the closest relatives of FIT28 are OE48.2, '' UCMA 17988, ZA5.3, OHA11, a390, P42, PS14 and Ps 9-14, within the subgroup of the lineage. The genome size of FIT28 is about 6.7 Mb with 59.09 mol% DNA G+C content. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values calculated from the genomic sequences of FIT28, and the closely related OE48.2 are 95.23 and 63.4 %, respectively. Biochemical, metabolic and chemotaxonomic studies further support our proposal that sp. nov., should be considered a novel species of the genus . Hence, the type strain FIT28 (=LMG 32353=DSM 112698) has been deposited in public cell-type culture centres.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): average nucleotide identity , digital DNA–DNA hybridization , endophyte , Iris germanica , multilocus sequence analysis , Pseudomonas and type-strain
Funding
This study was supported by the:
  • Ministerio de Ciencia, Innovación y Universidades (Award RTC-2017-6431)
    • Principle Award Recipient: AlbertFerrer
© 2022 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005268
2022-03-21
2024-05-01
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Pseudomonas germanica sp. nov., isolated from Iris germanica rhizomes
Int J Syst Evol Microbiol 72, 005268 (2022); https://doi.org/10.1099/ijsem.0.005268
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