1887

Abstract

A Gram-stain-positive, facultative anaerobic, motile and rod-shaped bacterial strain, DG-18, was isolated from desert soil sampled at the Kubuqi Desert in Inner Mongolia, China. Strain DG-18 grew at 4–40 °C (optimum, 25–30 °C), at pH 8.0–10.0 (optimum, pH 9.0) and with 0–8.0 % (w/v) NaCl (optimum 2.0%). 16S rRNA gene sequence analysis placed strain DG-18 within the genus of the family with DSM 8723 (98.2%), JSM 099021 (97.6%), DSM 8719 (97.4%), 18C (96.6 %) and NBRC 15565 (96.5%) as its closest relatives. The major respiratory quinone of strain DG-18 was MK-7 and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Its major fatty acids were iso-C and iso-C 10. The genomic DNA G+C content of strain DG-18 was 38.7 mol% based on total genome calculations. The average nucleotide identity score between the genomic sequence of strain DG-18 and that of DSM 8723 was 76.7 %. The Genome-to-Genome Distance Calculator showed that the DNA–DNA hybridization value for strain DG-18 and DSM 8723 was 21.8%. Based on the polyphasic taxonomic data, strain DG-18 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is DG-18 (=GDMCC 1.17773=KCTC 43170).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award NSFC, grant number 31070002)
    • Principle Award Recipient: JianliZhang
  • National Key Research and Development Program of China (Award 2016YFC0501302)
    • Principle Award Recipient: JianliZhang
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2022-02-23
2022-05-18
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