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Volume 72, Issue 2

gen. nov., sp. nov., a novel actinobacterial strain isolate from a Portuguese solar saltern and proposal of fam. nov. and ord. nov. No Access

Abstract

A novel micro-organism designated AS10 was isolated from dry salt collected from Aveiro saltern in the north of Portugal. Cells were Gram-stain-positive, non-motile, non-endospore-forming, rod-shaped and aerobic. Strain AS10 was characterized by long filaments of rod-shaped cells, presenting also coccoid cellular forms at the end of the filaments, unveiling some pleomorphism. Rod-shaped cells varied from 0.3 to 0.6 µm wide and from 0.6 to 2 µm long. Growth of AS10 occurred at 15–40 °C (optimum, 20–30 °C), 0–10% (w/v) NaCl (optimum, 2%) and pH 4.5–11.0 (optimum, pH 8.0–11.0). The peptidoglycan type was A1ϒ-type with 3-hydroxy-diaminopimelic acid. The major fatty acids were C, iso-C, C and C. The major respiratory quinone was MK-9(H). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AS10 was similar to actinobacterial members of the class , with ANL-iso2 being the closest relative the species with a sequence pairwise similarity of 91.21%. Average nucleotide identity, average amino acid identity and DNA–DNA hybridization values between strain AS10 and ANL-iso2 were 71.34, 53.57 and 18.90%, respectively. The genome DNA G+C content of AS10 was 71.8 mol%. Based on genomic, phylogenetic, phenotypic and chemotaxonomic studies, we describe a new species of a novel genus represented by strain AS10 (=LMG 31937=CECT 30148) for which we propose the name gen. nov., sp. nov. We also propose that this organism represents a new family named fam. nov. of a novel order named ord. nov.

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  • Accepted:
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Keyword(s): Actinobacteria , new taxon , Salsipaludibacter albus , Salsipaludibacteraceae , Salsipaludibacterales and saltern
Funding
This study was supported by the:
  • Fundação para a Ciência e a Tecnologia (Award UIDB/04539/2020)
    • Principle Award Recipient: LucianaAlbuquerque
  • Fundação para a Ciência e a Tecnologia (Award NORTE-01-0145-FEDER-000035)
    • Principle Award Recipient: NotApplicable
  • Fundação para a Ciência e a Tecnologia (Award SFRH/BD/125527/2016)
    • Principle Award Recipient: EduardaAlmeida
© 2022 The Authors
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2024-04-26
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Salsipaludibacter albus gen. nov., sp. nov., a novel actinobacterial strain isolate from a Portuguese solar saltern and proposal of Salsipaludibacteraceae fam. nov. and Salsipaludibacterales ord. nov.
Int J Syst Evol Microbiol 72, 005228 (2022); https://doi.org/10.1099/ijsem.0.005228
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