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Volume 72, Issue 3

sp. nov., isolated from marine sponge () No Access

Abstract

A flagella bacterium, designated strain 202IG2-18 was isolated from a marine sponge from Ulleung-do in the Republic of Korea. Cells were Gram-stain-negative, motile, aerobic, rod-shaped and non-pigmented. The strain was able to grow at pH 5.5–9.5 (optimum, pH 7.5), in the presence of 1–5 % (w/v) NaCl (optimum, 3 %, w/v) and at 18–30 °C (optimum, 30 °C). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 202IG2-18 belonged to the family , and was most closely related to [] NRRL B-41466 (97.9 %), followed by KCCM 43304 (97.1 %), KCTC 62318 (96.3 %) and KCTC 22492 (96.2 %). The predominant fatty acids were iso-C (25.7 %), C 8 (13.5 %), summed feature 3 (C 7 and/or C 6, 12.7 %), iso-C (10.4 %) and C (9.6 %). The only detected respiratory quinone was ubiquinone Q-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, three unidentified glycolipids, two unidentified aminophospholipids, an unidentified phospholipid and an unidentified aminolipid. The G+C content of the genomic DNA was 39.8 mol%. The average nucleotide identity values compared to all other related species was below 72.8 % and digital DNA–DNA hybridization values were 21.1–22.3 %, all below the threshold for bacterial species delineation. Phenotypic, phylogenetic, genomic and chemotaxonomic characteristics showed that strain 202IG2-18 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 202IG2-18 (=KACC 22256=LMG 32203).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene sequence , Parashewanella hymeniacidonis , polyphasic taxonomy and sponge
Funding
This study was supported by the:
  • National Research Foundation of Korea (Award 2021R1I1A3046479)
    • Principle Award Recipient: Jin-SookPark
  • National Marine Biodiversity Institute of Korea (Award 2021M01100)
    • Principle Award Recipient: Jin-SookPark
© 2022 The Authors
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Parashewanella hymeniacidonis sp. nov., isolated from marine sponge (Hymeniacidon sinapium)
Int J Syst Evol Microbiol 72, 005226 (2022); https://doi.org/10.1099/ijsem.0.005226
/content/journal/ijsem/10.1099/ijsem.0.005226
/content/journal/ijsem/10.1099/ijsem.0.005226
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