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Abstract

An investigation of the diversity of 1-aminocyclopropane-1-carboxylate deaminase producing bacteria associated with camel faeces revealed the presence of a novel bacterial strain designated C459-1. It was Gram-stain-negative, short-rod-shaped and non-motile. Strain C459-1 was observed to grow optimally at 35 °C, at pH 7.0 and in the presence of 0 % NaCl on Luria–Bertani agar medium. The cells were found to be positive for catalase and oxidase activities. The major fatty acids (>10 %) were identified as iso-C, summed feature 3 (C 6 and/or C 7) and iso-C 3-OH. The predominant menaquinone was MK-7. The major polar lipids consisted of phosphatidylethanolamine, one sphingophospholipid, two unknown aminophospholipids, three unknown glycolipids and five unknown lipids. The genomic DNA G+C content was 40.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain C459-1 was affiliated with the genus and had the highest sequence similarity to h337 (97.0 %) and HER1398 (95.6 %). The average nucleotide identity and digital DNA–DNA hybridization values between strain C459-1 and h337 were 83.8 and 33.8 %, respectively. Phenotypic characteristics including enzyme activities and carbon source utilization differentiated strain C459-1 from other species. Based on its phenotypic, chemotaxonomic and phylogenetic properties, strain C459-1 represents a novel species of the genus , for which the name sp. nov. is proposed, with strain is C459-1 (CGMCC 1.18716=KCTC 82381) as the type strain.

Funding
This study was supported by the:
  • Recruitment Program of Global Experts (Award YNWR-CYJS-2019-042)
    • Principle Award Recipient: MingHe Mo
  • China Tobacco Guizhou Industrial (Award 2021XM12)
    • Principle Award Recipient: MingHe Mo
  • China Tobacco Yunnan Industrial Corp (Award 2021530000241006)
    • Principle Award Recipient: MingHe Mo
  • Applied Basic Research Key Project of Yunnan (Award 202001BB050072,2019ZG0090)
    • Principle Award Recipient: LiMa
  • Major Research Plan (Award 31960022, 32170131, 31870091)
    • Principle Award Recipient: MingHe Mo
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/content/journal/ijsem/10.1099/ijsem.0.005215
2022-01-31
2024-12-05
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