1887

Abstract

Two Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and non-motile strains (LM13S and JZCK2) were isolated from hypersaline lakes in China. The colonies of both strains were yellow-pigmented and convex. Both strains could grow at 4–34 °C, pH 6.5–9.0 and with 1.0–13.0 % (w/v) NaCl. Comparisons based on 16S rRNA gene sequences showed that strains LM13S and JZCK2 share less than 98.3 % similarity with species of the genus . The phylogenetic tree reconstructed based on 16S rRNA gene sequences also showed that species are the most closely related neighbours of strains LM13S and JZCK2. The sequenced draft genome sizes of strains LM13S and JZCK2 are 4.06 and 4.22 Mbp with G+C contents of 37.0 and 37.8 mol%, respectively. The phylogenomic tree reconstructed using the Up-to-date Bacterial Core Gene set pipeline also demonstrated that both strains belong to the genus . The calculated pairwise average nucleotide identity values and digital DNA–DNA hybridization values between strains LM13S and JZCK2 and species were less than 86.4 and 32.0 %, respectively. The respiratory quinone in both strains was MK-6. Their major fatty acids were iso-C, iso-C, C 10, iso-C, anteiso-C, iso-C and C 10. Their major polar lipids included phosphatidylethanolamine, one unidentified lipid and one unidentified aminolipid, but strain LM13S also contained one more unidentified aminolipid, one more unidentified lipid and one unidentified phospholipid. Combining the above descriptions, strains LM13S and JZCK2 should represent two independent novel species of the genus , for which the names sp. nov. (type strain LM13S=GDMCC 1.2643=KCTC 82861) and sp. nov. (type strain JZCK2=GDMCC 1.2621=KCTC 82862) are proposed.

Funding
This study was supported by the:
  • Youth Innovation Promotion Association of the Chinese Academy of Sciences (Award 2014273)
    • Principle Award Recipient: PengXing
  • National Natural Science Foundation of China (Award 31670505)
    • Principle Award Recipient: PengXing
  • National Natural Science Foundation of China (Award 31722008)
    • Principle Award Recipient: PengXing
  • the Second Tibetan Plateau Scientific Expedition and Research (STEP) program (Award 2019QZKK0503)
    • Principle Award Recipient: PengXing
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2022-01-25
2022-05-18
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