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Volume 72, Issue 1

sp. nov., isolated from the kelp No Access

Abstract

A Gram-stain-positive, aerobic, non-sporulating, yellow-pigmented and rod or cocci-shaped bacterium, designated Arc0846-15, was isolated from the kelp . Strain Arc0846-15 was found to grow at 16–35 °C (optimum, 28 °C), at pH 6.0–9.5 (optimum, 7.0) and in the presence of 0–6 % (w/v) NaCl (optimum, 2 %). Cells were positive for catalase and negative for oxidase activity. Phylogenetic analyses, based on 16S rRNA gene sequence comparisons, revealed that the nearest phylogenetic neighbour strains of strain Arc0846-15 were 01 Gi-040 (96.2 %), K22-20 (96.1 %) and HKI 0124 (95.2 %). Based on phylogenomic analysis, the average nucleotide identity values between strain Arc0846-15 and the neighbour strains were 69.8, 69.7 and 69.8 %, respectively; the digital DNA–DNA hybridization values between strain Arc0846-15 and its three closest neighbour strains were 18.8, 19.1 and 19.3 %, respectively. The predominant menaquinone was MK-8 (H). The dominant cellular fatty acids were C 8, iso-C, iso-C and C. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, glycolipid, one unidentified aminolipid and four unidentified lipids. The DNA G+C content of strain Arc0846-15 was 61.6 mol% based on the whole genome sequence. Based on the phylogenetic and phenotypic characteristics, strain Arc0846-15 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed, with Arc0846-15 (=KCTC 49655=MCCC 1K06093) as the type strain.

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  • Accepted:
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Keyword(s): Actinobacteria , Ornithinimicrobiaceae , Ornithinimicrobium laminariae and polyphasic taxonomy
Funding
This study was supported by the:
  • State Key Laboratory of Microbial Technology Open Projects Fund (Award M2020-03)
    • Principle Award Recipient: Wen-RuiCao
  • Fundamental Research Funds for Central Universities of the Central South University (Award 2019HW022)
    • Principle Award Recipient: Ying-JieLi
  • National Natural Science Foundation of China (Award 41976202)
    • Principle Award Recipient: Ming-YuJiang
  • Strategic Priority Research Program of the Chinese Academy of Sciences (Award XDB42000000)
    • Principle Award Recipient: NotApplicable
  • Open Fund of the Key Laboratory of Marine Geology and Environment, Chinese Academy of Sciences (Award MGE2020KG14)
    • Principle Award Recipient: Wen-RuiCao
  • Natural Science Foundation of Shandong Province (Award ZR2020MD088)
    • Principle Award Recipient: Wen-RuiCao
© 2022 The Authors
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2022-01-19
2024-10-10
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Ornithinimicrobium laminariae sp. nov., isolated from the kelp Laminaria japonica
Int J Syst Evol Microbiol 72, 005187 (2022); https://doi.org/10.1099/ijsem.0.005187
/content/journal/ijsem/10.1099/ijsem.0.005187
/content/journal/ijsem/10.1099/ijsem.0.005187
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