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Abstract

Strain NIBR12 (=KACC 22094=HAMBI 3739), a novel Gram-stain-negative, obligate aerobic, non-spore-forming, non-motile and coccobacillus-shaped bacterium, was isolated from a cyanobacterial sample culture ( NIBRCYC000000452). The newly identified bacterial strain grew optimally in modified Reasoner's 2A medium under the following conditions: 0 % (w/v) NaCl, pH 7.5 and 35 °C. Phylogenetic analysis using the 16S rRNA gene sequence confirmed that strain NIBR12 belongs to the genus , with its closest neighbours being SHET (98.8%), RB-3 (97.7%), “ R-30 (95.7 %) and H-8 (95.0 %). Genomic comparison of strain NIBR12 with type species in the genus was conducted using digital DNA–DNA hybridization, average nucleotide identity and average amino acid identity analyses, resulting in values of ≤53.7, ≤93.7 and ≤96.1 %, respectively. The genomic DNA G+C content of strain NIBR12 was 70.9 mol%. The major fatty acids of strain NIBR12 were summed feature 8 (C7 and/or C 6) and summed feature 3 (C 6/C 7). Q-9 was its major respiratory quinone. Moreover, the major polar lipids of strain NIBR12 were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. Based on our chemotaxonomic, genotypic and phenotype analyses, strain NIBR12 is identified as represeting a novel species of the genus , for which the name sp. nov. is proposed.

Funding
This study was supported by the:
  • national institute of biological resources (Award NIBR202002108)
    • Principle Award Recipient: WoojunPark
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2021-12-16
2022-01-17
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