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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 72, Issue 2

sp. nov. and sp. nov., new members in the genus No Access

Abstract

Four novel bacterial strains (zg-ZUI122/zg-ZUI10 and zg-ZUI227/zg-ZUI100) were isolated from the intestinal contents of and characterized using a polyphasic approach. Cells were Gram-stain- and catalase-positive, urease- and oxidase-negative. Strains grew optimally at 28–30 °C, pH 7.0, with 0.5 % NaCl (w/v). A comparative analysis of 16S rRNA gene sequences revealed that strain pairs zg-ZUI122/zg-ZUI10 and zg-ZUI227/zg-ZUI100 belonged to the genus and were most closely related to DSM 20133, with similarities of 99.6 and 99.5 %, respectively. This was further confirmed by phylogenetic analyses based on the 16S rRNA gene and genome sequences. The digital DNA–DNA hybridization and average nucleotide identity values between the two new type strains (zg-ZUI122 and zg-ZUI227) and other species in the genus were 20.0–24.4/77.2–83.4% and 19.9–25.1/77.1–83.4%, all below the thresholds. The major cellular fatty acids detected in the two novel species included -C and -C; the predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. MK-8(H) (77.3%) was the predominant respiratory quinone detected in strain zg-ZUI122, while MK-8(H) (53.7%) and MK-9(H) (46.3%) were detected in strain zg-ZUI227. The shared cell-wall amino acids detected in the two novel species were alanine, glutamic acid and lysine; the shared whole cell wall sugars consisted of galactose, mannose and ribose. All these analyses concluded that these four strains represent two different novel species in the genus , for which the names sp. nov. (zg-ZUI122 = GDMCC 1.2502 = KCTC 49677) and sp. nov. (zg-ZUI227 = GDMCC 1.2500 = KCTC 49676) are proposed.

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Keyword(s): ANI , Arthrobacter , dDDH , novel species and phylogenetic analysis
© 2022 The Authors
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2022-02-01
2024-04-26
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Arthrobacter sunyaminii sp. nov. and Arthrobacter jiangjiafuii sp. nov., new members in the genus Arthrobacter
Int J Syst Evol Microbiol 72, 005181 (2022); https://doi.org/10.1099/ijsem.0.005181
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