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Volume 72, Issue 1

sp. nov., an endophytic actinobacterium isolated from the root of (L.) No Access

Abstract

The polyphasic taxonomic study of a novel endophytic actinobacterium strain (CA2R105) was carried out. The strain formed fragmented substrate mycelium and showed chemotaxonomic properties typical of members of the genus i.e. the presence of mycolic acid and MK-8 (H) in its cells. Strain CA2R105 exhibited the highest 16S rRNA gene sequence similarity to NBRC 101359 (99.2%). The genome-based taxonomic analysis revealed low average nucleotide identity- and digital DNA–DNA hybridization values (<93.7, and <65.2%, respectively) to its closest relative. Moreover, many different phenotypic characteristics were observed between strain CA2R105 and all related -type strains. This taxonomic evidence suggested that strain CA2R105 should be judged as representing a novel species of the genus and the name, sp. nov. is proposed. The type strain is CA2R105 (=TBRC 11247=NBRC 114292).

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Keyword(s): 16S rRNA gene , Coffea arabica (L.) , endophytic actinobacterium and Nocardia
Funding
This study was supported by the:
  • Faculty of Science, King Mongkut’s Institute of Technology Ladkrabang (Award RA/TA-2562-M-021)
    • Principle Award Recipient: AchararakNammali
  • King Mongkut’s Institute of Technology Ladkrabang Research Fund (Award KREF046301)
    • Principle Award Recipient: ChittiThawai
© 2022 The Authors
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2022-01-17
2024-04-26
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Nocardia coffeae sp. nov., an endophytic actinobacterium isolated from the root of Coffea arabica (L.)
Int J Syst Evol Microbiol 72, 005180 (2022); https://doi.org/10.1099/ijsem.0.005180
/content/journal/ijsem/10.1099/ijsem.0.005180
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