1887

Abstract

Three novel, anaerobic, Gram-positive bacteria were isolated from the eggshell of two separate white leghorn chicken flocks and the ileum of a healthy pig, and designated MMM721, ISU324 and PIG517 respectively. Cells were pleomorphic and capable of forming long chains of rods or coccoid clusters. Phylogenetic analysis of the 16S rRNA gene sequences identified these strains to be within the genus , of which only one species, , has been formally described. However, whole genome sequencing of novel isolates returned a digital DNA–DNA hybridization value of 22.5 % and average nucleotide identity (ANI) values of 76.4 % (ANIb) and 86.0 % (ANIm), indicating divergence between the type strain MMM721 and , suggesting the strains represented a novel species. The major fatty acid methyl esters of strain MMM721 were C, C ω7 and C. The strains mainly produced the volatile fatty acid lactate, along with smaller amounts of acetate and butyrate. Together, these data indicate that MMM721, along with ISU324 and PIG517, represent a novel species within the genus . We propose the name sp. nov. for the species. The type strain is MMM721 (=ATCC TSD-238=CCUG 74757).

Keyword(s): anaerobe , poultry , swine and turicibacter
Funding
This study was supported by the:
  • Agricultural Research Service
    • Principle Award Recipient: JoelJacob Maki
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2022-01-27
2022-05-18
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